Haematoxylin

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Haematoxylin
Haematoxylin.svg.png
Names
IUPAC name
7,11b-Dihydroindeno[2,1-c]chromene-3,4,6a,9,10(6H)-pentol
Properties
C16H14O6
Molar mass 302.28 g/mol
Appearance White to yellowish solid
Melting point 120 °C (248 °F; 393 K) (decomposes)
Boiling point Decomposes
Slightly soluble
Hazards
Safety data sheet Sigma-Aldrich
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Haematoxylin also called hematoxylin, natural black 1 or C.I. 75290 is a compound extracted from the heartwood of the logwood tree. Hematoxylin is a basic / positive compound that binds to and forms salts with acidic, or basophilic, compounds containing negative charges (such as DNA and RNA which are acidic/negative because the nucleic acid building blocks that come off the phosphate backbone are negatively charged) and stains them dark blue or violet. Haematoxylin and eosin together make up haematoxylin and eosin stain, one of the most commonly used stains in histology. This type of stain is a permanent stain as opposed to temporary stains (e.g. iodine solution in KI).

Properties

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Preparation

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References

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