Sciencemadness Discussion Board

Isozyme picture wrong

DNA - 12-1-2010 at 13:48

Can anyone see what is wrong in this picture?
And also explain why it is wrong please?


isozyme.jpg - 27kB

chemoleo - 12-1-2010 at 15:19

There's one cell type here that is different to all the others. Which one is it?

Therein lies the answer! Or so I suspect.

See http://en.wikipedia.org/wiki/Lactate_dehydrogenase , look what it does and what it needs for activity ..and go and figure!

Le'ts see what you come up with :)

[Edited on 12-1-2010 by chemoleo]

DNA - 13-1-2010 at 06:14

LDH-1 (4H) - in the heart
LDH-2 (3H1M) - in the reticuloendothelial system
LDH-3 (2H2M) - in the lungs
LDH-4 (1H3M) - in the kidneys
LDH-5 (4M) - in the liver and striated muscle

Are all there but the leukocyte isn't.
But rather I'dd think there should be a big dash at H4 instead of the very little one.

I already need to answer for tomorrow morning to get extra points for my exam.
Could you please tell me what's wrong and if my conclusion is correct?

[Edited on 13-1-2010 by DNA]

[Edited on 13-1-2010 by DNA]

chemoleo - 13-1-2010 at 16:47

You left out the red blood cells.

I haven't researched this properly, that's your job - but consider this:

Erythrocytes are anucleate, they don't have mitochondria, ER and Golgi.
The important bit is the mitochondria - no mitochondria, no glycolysis, Krebs cycle, electron transport, mitochondrial proton pumps, et cetera.
LDH needs NADH, where would it come from if there aren't mitochondria to generate it?

I might be wrong because like I said, I haven't researched it, but erythrocytes are so dissimilar to all the other cell types (every other one of these have all the cellular organelles) that I don't think these factors are a coincidence.

Think/read about it with this in mind, and good luck with your decision!