magnus454 - 4-10-2011 at 20:29
Has anybody used plain gelatin or agar to make a gradient for centrifuging? If so what formula did you use to get the different densities?
phlogiston - 21-10-2011 at 16:01
What do you want to do with it, exactly? Probably, you want to make a density gradient, but it doesn't seem very suitable for that. You would need to
dissolve a lot to get any relevant density increase, and then the holes in the maze are going to be too small for larger biomolecules or organelles to
pass through.
If you something cheap and readily available, use sucrose (common table sugar). I've separated liposomes and organelles with it with good success.
anonymous201 - 21-10-2011 at 16:16
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