Sciencemadness Discussion Board

preparative TLC plates with custom layer

Cloner - 21-9-2006 at 04:04

When I run a column, my first choice is silica. If that gives trouble, cellulose is a good second. However, its always a long time running a column especially for sample amounts.

This is why I am using silica TLC plates with a layer that you can scrape off and investigate the spot. This works well enough with most materials. But i'd like to take a used plate, remove everything and make a cellulose layer on it for those materials that don't work on silica. Does anyone have experience making TLC plates with cellulose or anything else that doesn't involve the heavy inorganics?

Eclectic - 21-9-2006 at 05:03

Maybe you could just stick a piece of filterpaper to a sheet of glass?

solo - 21-9-2006 at 05:27

Here is a book which may be of help, ..............solo

http://rapidshare.de/files/33914791/thin_Layer_Chrmatography...

filter papaer separations

chemrox - 18-1-2007 at 23:29

I second the other reply that said why not stick filter paper to glass slides except that why use glass at all?

I do quick checks with filter paper cut to fit in a test tube. I hang it from a paper clip inserted into a cork and suspend the spotted paper in the small reservoir of solvent at the bottom.