How to make Cats Glow in the Dark

Before reading this I suggest you learn, How to Make a HIV Vector .

This time you need to use the Feline Immunodeficiency Virus , F.I.V.

The Genome can be found F.I.V. Genome NBCI

Next, you find the gene for Bio-Luminescence, you can choose one of three colors Red,Green, or Blue. This will be green because this gene is from the Japanese firefly.



Green Bio-luminesence Protein Japanese Firefly

Now we want to make the code for this Gene with a LTR 5' Primer on the End and LTR 3' on the Beginning using Viral Gene Synthesis .


Then Use the F.I.V. Virus's ENV gene long with Gag-Pol gene, this will be the machinery we use to transfer the Bio-Luminescence Gene

Put the genes into T-Cells to make a Packing Transfer Plasmid.





Transfect the Set of cells in the embryo or other cells that you want to glow in the dark green, which means either add it to the blood of the mother or directly to the cells.



When that Mother cat gives birth her kittens will glow in the dark just like a Japanese Firefly having this gene added to the kittens.