RJR
Harmless
Posts: 1
Registered: 5-1-2021
Member Is Offline
|
|
Need help identifying 2 proteins in a research paper, cannot find them in protein databases
The first one identified is FST 291. FST 288 is apparently a known protein with lots of literature https://www.ebi.ac.uk/pdbe/entry/pdb/5jhw/protein/2
But I couldn't find any mention of FST 291
The other one is FST-315. I found an isoform of human follistatin, which has 317 AAs which is close but not quite a match. I also found an
activin-follistatin complex with 315 AAs, but I'm not sure if that's a match https://www.ncbi.nlm.nih.gov/protein/2P6A_C
Here's the figure with the proteins (in part a)
Thanks in advance!
|
|
|
zinc finger
Harmless
Posts: 25
Registered: 30-7-2019
Location: Germany
Member Is Offline
|
|
So this seems to be the paper you are referring to? https://www.nature.com/articles/s41598-019-47818-w
Here you can find the structural and sequence data for follistatin 315: https://www.rcsb.org/sequence/2P6A
You can verify that it is indeed the same Protein by comparing the annotated features lengths.
The question about the follistatin 291 is more difficult. As I understand it they assigned the name FST291 to the remining rest of FST315 after
removing the CD. When searching for ACE-083 you get much more results. So I would assume, that it is just the sequence of FST315 until the 291th aa.
"ACE-083 differs from native FST315 by nearly complete removal of the C-terminal domain (CD) and by attachment to
an immunoglobulin Fc domain, which results in dimerization of the follistatin polypeptide."
(Follistatin-based ligand trap ACE083 induces localized hypertrophy
of skeletal muscle with functional
improvement in models of
neuromuscular disease
R. S. Pearsall)
|
|
|
Metacelsus
International Hazard
Posts: 2531
Registered: 26-12-2012
Location: Boston, MA
Member Is Offline
Mood: Double, double, toil and trouble
|
|
Quote: Originally posted by zinc finger  | So I would assume, that it is just the sequence of FST315 until the 291th aa.
"ACE-083 differs from native FST315 by nearly complete removal of the C-terminal domain (CD) and by attachment to
an immunoglobulin Fc domain, which results in dimerization of the follistatin polypeptide." |
Yes, this is correct. FST291 likely does not occur naturally, which is why it's not in databases.
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
