Sciencemadness Discussion Board
Not logged in [Login - Register]
Go To Bottom

Printable Version  
 Pages:  1  2  
Author: Subject: Luminous Glowing Bacteria - Homemade!!
Esplosivo
National Hazard
****




Posts: 492
Registered: 7-2-2004
Location: Mediterranean
Member Is Offline

Mood: Quantized

[*] posted on 9-8-2004 at 12:00


I checked the fish right now. I could not see any clear luminous colonies, but on the part of the fish which was left outside the water I could see a very faint light. I am not sure that these could be the bacteria. So what I will do is that tomorrow I will buy the necessary chemicals and try innoculating a medium with all the bacteria present on the surface of the fish as mentioned previously.

If I manage to collect just a couple of luminescent bacteria a colony will form and from this colony I could make millions of others.

Btw, I tried taking a picture with my cheap camera but nothing was visible. The camera has no aperture settings so the ammount of light entering the cam cannot be controled. Anyway, I will try enhancing the pic.

Edit: The growth of the bacteria will be postponed since I cannot obtain the yeast / beef extract till next month. Till then I will try acquiring two different species of fish, and probably even a squid or octopus.

[Edited on 11-8-2004 by Esplosivo]




Theory guides, experiment decides.
View user's profile Visit user's homepage View All Posts By User
gorkem
Harmless
*




Posts: 6
Registered: 20-12-2004
Member Is Offline

Mood: gorkem

[*] posted on 21-12-2004 at 13:00
lightning bacteria


we want to produce lightining bacteria in the college environment but we fail.do you know how to do it?

You might want to read this very thread first. Chemoleo:)

[Edited on 21-12-2004 by chemoleo]
View user's profile View All Posts By User This user has MSN Messenger
vulture
Forum Gatekeeper
********




Posts: 3332
Registered: 25-5-2002
Location: France
Member Is Offline


[*] posted on 21-12-2004 at 13:35


Read the thread first and DO NOT crossposts three times!!

One more stunt like this and your posting ability will be gone with the wind.




One shouldn't accept or resort to the mutilation of science to appease the mentally impaired.
View user's profile View All Posts By User
Madandcrazy
Hazard to Others
***




Posts: 117
Registered: 11-5-2005
Member Is Offline

Mood: annoyed

[*] posted on 10-7-2005 at 07:20


Vulture you meant the crosspost to chemolos metabolic pathway topic ?

I don`t know the method isolating lightning substances from insects would walk ?
(wings of chafers or some tropic insects)



[Edited on 10-7-2005 by Madandcrazy]
View user's profile View All Posts By User
Ravenstein
Harmless
*




Posts: 1
Registered: 9-8-2005
Member Is Offline

Mood: Wah Lao SO SHAG

shocked.gif posted on 9-8-2005 at 00:30
Help!


Hi all, I am from Singapore and and trying to do this ... I bought some octopus and and letting them rot in my fridgerator half submerged in sea water. Its been nearly 18 hrs since i started. At first i did'nt read the things well and left my octopus outside for about 12 hrs and earlier this morning, i placed them in my fridgerator after i realised what happened. Will this affect the photobacterium?

For the tryptone, i am in early high school and am not very sure how to obtain this, early in this thread someone said any protein extract should work... is it true? and if not, what should i use.

For the medium can i use the LM and just dont add agarose gel?

How fast does photobacterium grow?

How long will it take to get about 100ml of the bacteria?

How do i grow the bacteria in such a way i can get alot of it quickly?

In singapore, anyone know where i can buy petri dishes and the rest of the ingridents?
View user's profile View All Posts By User
Bugger
Harmless
*




Posts: 1
Registered: 31-8-2005
Member Is Offline


[*] posted on 31-8-2005 at 14:43


I've got a question:

I live in an inland state in a town where there's no fresh sea-fish available; everything's either frozen or already cut up and probably washed and cleaned. Is there no other way I could get a sample of this glowing bacteria from nature then? Would aquarium water from a tank w/ live rock contain the bacteria, for instance?

Thanks for any help!
View user's profile View All Posts By User
sparkgap
International Hazard
*****




Posts: 1222
Registered: 16-1-2005
Location: not where you think
Member Is Offline

Mood: equilibrated

[*] posted on 1-9-2005 at 02:57


OK, let's review what has been said thus far...

-P. phosphoreum is a *marine* organism.

-Freshly-caught marine animals *may* carry the microbe. Store-bought animals may have already been cleaned and as such may have little, if any, microbe present.

-Yeast lysate and tryptone are possible protein sources. (There should be bakeries nearby that have yeast, FCOL).

-Use gelatin or agar as a medium. Shouldn't be difficult if you live in a tropical country... they're extensively used in a good number of Oriental deserts.

sparky (~_~)




"What's UTFSE? I keep hearing about it, but I can't be arsed to search for the answer..."
View user's profile View All Posts By User
Xque
Harmless
*




Posts: 20
Registered: 8-11-2005
Location: Europe
Member Is Offline

Mood: :S

[*] posted on 17-11-2005 at 06:51


You might want to look into Pseudomonia ssp. too. There's several species which are flourescent when lacking iron, such as P. aeruginosa, P. flourescens , P. putida and P. syringiae. It seems Pseudomonia is pretty common.

I wouldn't mess too much with aeruginosa though, as it is a pathogen.
View user's profile View All Posts By User
nitro-genes
International Hazard
*****




Posts: 781
Registered: 5-4-2005
Member Is Offline


[*] posted on 12-1-2006 at 08:52


That's funny :) We did exactly the same experiment when I was a student. This was for a course in microbial ecology. We used "fresh fish" from the market nearby, but I've heard that fish from the market can be as old as two weeks :o

Maybe that was the reason that only one group actually got V. Phosphoreum. Our group wasn't able to spot any luminous colonies on the fish, so obtaining the right fish is the hard part I think.
We incubated the fish at 4 deg. C to deminish competition with other microorganisms. (V. phosphoreum can grow quite fast at 4 deg. C.) And looked for colonies twice a day...

Quite funny was that our professor told us that every now and then reports come in from terrified people that claim to have eaten "radiation contaminated" seafood. ;) The bacterium however is of no harm IIRC.

I wonder what the reason is for it's dissapearance after the fish gets older. V. phosphoreum is facultative anaeroob and able to grow at a wide range in temperature. Maybe because of competition with other faster growing microorganisms?

Oh, and an idea for people that need yeast extract...There are these nutritional yeast pills that are told to be rich in Vitamin B. I believe they are mayde of pressed and dried cells of S. cerevisiae siccum :D
View user's profile View All Posts By User
Esplosivo
National Hazard
****




Posts: 492
Registered: 7-2-2004
Location: Mediterranean
Member Is Offline

Mood: Quantized

[*] posted on 12-1-2006 at 09:01


That's nice to hear. So you did manage to grow colonies on the fish. I have tried with two freshly caught fish (caught by a friend that very moment and kept in sea water - fresher than that is impossible) but with no apparent success, except on the second time where I think there were visible light emitting colonies which were very faint.

Could you please give us more detail regarding this experiment. You said you incubated the fish at 4degC - for how long? When did the first colonies become visible? Did you incubate the fish in sea water? Do you have any idea of what species of fish did the bacteria actually grow on (the common name is sufficient)? Thank you.

[Edited on 12-1-2006 by Esplosivo]




Theory guides, experiment decides.
View user's profile Visit user's homepage View All Posts By User
nitro-genes
International Hazard
*****




Posts: 781
Registered: 5-4-2005
Member Is Offline


[*] posted on 12-1-2006 at 13:01


I'm sorry if I made the impression that luminous bacteria are specific for some species of fish... What I meant was that you need a fish with the "right" fressnes. I/m saying "right" because I haven no clue why the colonies show up in some cases as in others it doesn't. Could be a lot of things, but In our experiment we used plain cod. The group that had succes found colonies after less than two days @ 4 deg C already.

The light is only emmited during the growth fase, so you should be able to see the luminescence quite quickly. That is probably also the reason why you must have very fresh fish.The bacteria will still be there, but not emmitting any light anymore!

Luminous bacteria are present in almost all seas at any depth and can be found in almost every marine organism. Of course there are more species of luminous bacteria, so I don't know if this is the case for V. phosphoreum. But seen it's large temperature range in which it can grow, and the commenly found niche it occupies, it is quite likely.

In the attachment is a pdf that is very simple, it contains almost every information you need, like media, growth conditions and background information.


[Edited on 12-1-2006 by nitro-genes]

Attachment: Luminous bacteria.pdf (186kB)
This file has been downloaded 1273 times

View user's profile View All Posts By User
Vitus_Verdegast
National Hazard
****




Posts: 297
Registered: 5-12-2004
Location: Ottoman Empire
Member Is Offline

Mood: tea time

[*] posted on 8-4-2006 at 09:30
TCBS agar preparation


Thiosulphate Citrate Bile Salts Agar (TCBS)

(Oxoid designation is Cholera Medium TCBS)


Basic ingredients

Peptone 10,0 g
Yeast extract 5,0 g
Sodium citrate 10,0 g
Sodium thiosulfate.5 H2O (Na2S2O3•5 H2O) 10,0 g
Sodium chloride (NaCl) 10,0 g
Ox gall powder 5,0 g
Iron (III) citrate (FeC6H5O7) 1,0 g
Sodium cholate 3,0 g
Sucrose 20,0 g
Thymol Blue solution (0,2%) 20,0 ml
Bromothymol Blue solution (0,2%) 20,0 ml
Agar 15,0 g

Distilled water 1,0 L
pH 8,6 ± 0,2


Preparation

Mix all ingredients and apply heat until all goes into solution. DO NOT STERILIZE USING AUTOCLAVE. Allow to cool to 45-50°C and distribute.


Notes:


from http://www.disknet.com/indiana_biolab/b041.htm:
Peptones are the most widely used source of nitrogen in microbial media. Some are made by cooking milk or meat products in acid, but most are made by incubating milk or meat with trypsin, pepsin, or other proteolytic enzymes to digest the protein to a mixture of amino acids, peptides, and polypeptides. Many microbes, called proteolytic, can digest proteins, but most can't. The choice of peptone is sometimes of importance.

Some water-based inks contain ox gall (bile) as a dispersant. Pure ox gall can be obtained from art supply stores (eg. Winsor & Newton brand).

Sodium cholate, a bile salt, is used as an anionic detergent.
View user's profile View All Posts By User
kazaa81
National Hazard
****




Posts: 368
Registered: 30-4-2004
Member Is Offline

Mood: ok

[*] posted on 9-6-2006 at 05:25


Maybe this is a little off-topic, but I would mention some dinoflagellates of the genre Noctiluca, that, when they're shaken, emits light.
Obviously, being monocellular organism of larger size, it would be more difficult to spot and grow as done with Photobacterium; it has been reported of some species that can reach 2mm of diameter...that's very big for a monocellular organism!

bioluminescent dinoflagellates at luxgene.com
http://www.bioart.co.uk/lux/dino.html

"Noctiluca" on wikipedia
http://en.wikipedia.org/wiki/Noctiluca

Noctiluca scintillans information pages
http://www.marbot.gu.se/SSS/dinoflagellates/Noctiluca_scinti...
http://www.nmnh.si.edu/botany/projects/dinoflag/Taxa/Nscinti...

Noctiluca scintillans photo
http://micro.magnet.fsu.edu/featuredmicroscopist/vanegmond/i...
http://micro.magnet.fsu.edu/featuredmicroscopist/vanegmond/i...
http://members.fortunecity.com/anemaw/noctiluca.jpg

The Subcellular Origin of Bioluminescence in Noctiluca miliaris
http://www.jgp.org/cgi/content/abstract/50/5/1429

[Edited on 9-6-2006 by kazaa81]
View user's profile View All Posts By User
JohnWW
International Hazard
*****




Posts: 2849
Registered: 27-7-2004
Location: New Zealand
Member Is Offline


[*] posted on 28-3-2007 at 02:10


What enzymes, and substrate, are involved in the chemoluminescence reactions? In the cases of fireflies and cave glow-worms, I understand it is luciferase and luciferin.
View user's profile View All Posts By User
bereal511
Hazard to Others
***




Posts: 162
Registered: 9-8-2005
Location: Madison, WI
Member Is Offline


[*] posted on 26-6-2007 at 20:58


Hmmm, I wonder if anyone was successful in growing and maintaining a culture of the luminous bacteria so far.

I just spent the past week conducting the procedure to allow growth of bacteria on fish, with great success. The fish I caught off the pier, a mackerel I believe, so it was as fresh as it was going to get. I immediately iced it to both kill and preserve the fish. I filled the container half way (about 250 mL) with ocean water and added about 5 grams of salt to ensure that only the luminous bacteria would be growing on the fish and to also make up for the addition of tap water from the ice. The container was left in another container which had ice water and was allowed to sit for 2 days.

I got a very strong glow in a completely dark room, and I just inoculated a 1000 mL solution of 20 grams sodium chloride, 5 grams of yeast extract that I prepared using a 50 mL of 5 molar sodium chloride lysing solution, 10 grams beef boullion (with delicious beef extract, yeast extract, salt, hydrolyzed proteins, and fatty acids), and 0.5 grams of magnesium sulfate. I hope by Thursday I can get some results up. I can't get a clear picture of the glowing fish on my digital camera, but I kept it outside to see if it'll have an even stronger glow. Updates soon!




As an adolescent I aspired to lasting fame, I craved factual certainty, and I thirsted for a meaningful vision of human life -- so I became a scientist. This is like becoming an archbishop so you can meet girls.
-- Matt Cartmill
View user's profile View All Posts By User
chemoleo
Biochemicus Energeticus
*******




Posts: 3005
Registered: 23-7-2003
Location: England Germany
Member Is Offline

Mood: crystalline

[*] posted on 27-6-2007 at 14:54


Fantastic, that is good news!
Just make long exposures (>1 sec) and I'm sure you'll get good images, and do post with reference objects! Even cooler if you can grow it in some sort of dish!
Well done!




Never Stop to Begin, and Never Begin to Stop...
Tolerance is good. But not with the intolerant! (Wilhelm Busch)
View user's profile View All Posts By User
bereal511
Hazard to Others
***




Posts: 162
Registered: 9-8-2005
Location: Madison, WI
Member Is Offline


[*] posted on 28-6-2007 at 09:01


I don't think my camera has a long exposure function. Which is unfortunate, because the bacteria was out-competed, so no more glow. I still have the inoculated culture medium though, so I'm placing my bets on that. I just wish I had isolated it on an agar plate first, but I have no plates. Hopefully the conditions will be good enough that the luminous bacteria aren't killed off by other bacteria. Otherwise I'll start over with a culture plate.



As an adolescent I aspired to lasting fame, I craved factual certainty, and I thirsted for a meaningful vision of human life -- so I became a scientist. This is like becoming an archbishop so you can meet girls.
-- Matt Cartmill
View user's profile View All Posts By User
Spargine
Harmless
*




Posts: 10
Registered: 19-10-2007
Location: uncertain
Member Is Offline

Mood: superposition

[*] posted on 6-12-2007 at 09:02


Has anyone had success with fairly inland fresh fish? I am thinking about attempting this and I am wondering if anyone else has had success.
View user's profile View All Posts By User
chemoleo
Biochemicus Energeticus
*******




Posts: 3005
Registered: 23-7-2003
Location: England Germany
Member Is Offline

Mood: crystalline

[*] posted on 16-12-2007 at 16:10


I don't think there are any phototrophic bacteria known that grow in fresh water...


Bereal - well next time make a glycerol stock, to conserve the bacteria! Shame you lost them :(
With a glycerol stock, you just dip a sterile needle into the first successful culture, which is then dipped into to sterile (heated prior to this) 50/50 glycerol/water.
Then you should be able to restart your growth as many times you wish!




Never Stop to Begin, and Never Begin to Stop...
Tolerance is good. But not with the intolerant! (Wilhelm Busch)
View user's profile View All Posts By User
ChrisWhewell
Hazard to Self
**




Posts: 66
Registered: 22-12-2009
Location: Austin
Member Is Offline


[*] posted on 25-12-2009 at 11:23


Quote: Originally posted by chemoleo  
I read something very interesting recently - I got a pdf but can't remember where I got it from. Anyway, here it goes:

Quote:
PASSENGERS on board cruise liners are sometimes startled by an eerie phosphorescent glow emitted by seawater flush lavatories. The explanation lies in luminous bacteria, which require oxygen for their light-generating reactions. Luminescent bacteria are present in all marine environments and develop abundantly on the surface of decaying fish. Their presence can be a problem — most of
the research on marine bioluminescence has been funded by the US Navy, embarrassed by glowing trails behind surface vessels, and more recently by bacterial interference
with the laser light used for submarine communications.


Upon searching some more:

The bacterium causing luminescence is Vibrio (Photobacterium) phosphoreum, it thrives between 20 and 30 deg C, and grows well at saline conditions. The bacterium requires oxygen to produce light, and the reaction roughly works as a luciferase (enzyme) - FADH2 (flavo-cofactor) complex, where light is emitted once it reacts with O2 and RCH2OH to form RCOOH and H2O. Light is emitted in the bluish green range (460–490 nm).

THen, at http://www.disknet.com/indiana_biolab/b203.htm it says:
It grows on glucose, mannose, fructose, or glycerol as a sole carbon source, and synthetic or real seawater as a growth medium.

ISOLATION :

Incubate fresh fish, squid, or octopus at 10-15 deg Celcius, half submerged in natural or synthetic seawater (I take it this shouldn't be sterile seawater! This will stink, so do it outside!). Examine at half-day or daily intervals for glowing colonies of bacteria (in total darkness after your eyes are dark adapted).

Streak these colonies on LM (Luminescence Medium). LM is synthetic or natural seawater containing per liter: 3 ml glycerol (glycerine), 5 gram yeast extract, 5 g tryptone, 1 g CaCO3 (powdered limestone or chalk) and 10 g of agar (per litre). For streaking, sterile toothpics can be used (i.e. soak them in ethanol and dry over flame, and use immediately after they cooled down)

I looked up agar, and it is a linear complex sugar made from beta-galactopyranose linked to 3,6-anhydro-L-galactopyranose. Basically, its just a polysaccharide/carbohydrate, or complex sugar. I should think any other complex sugar would do, such as starch, alginate (a linear complex sugar made from beta-D-mannuronic acid and alpha-L-guloronic acid) etc.

Regarding tryptone, this is a pancreatic digest of casein, a protein. I should think any protein extract should do, you will get that in Health shops/Fitness shops etc.
Yeast extract is similarly easy to get, it is basically a whole cell lysate of yeat, and thus contains vitamins, protein, DNA/RNA, lipids, minerals etc. Isn't Marmite a yeast extract?

Anyway, back to the isolation of Photobacterium phosphoreum!

So, once you have streaked luminous colonies onto the plates, you can either grow them at room temp. or at 4 deg C (fridge). With the latter, check every day for growth by looking at the plates in the dark!

For growth in liquid medium, just leave out the agar (whcih is the gelling agent).
So I would first grow the bacteria on the fish, and meanwhile prepare covered plates with the growth medium. Alternateively, use a small bottle that is sterile (Ethanol-rinsed) and poor in the hot growth medium. Let it cool down, and once at room temperature, add a bit of the luminous colonies from the fish. Only very very little is needed! Even if you dont see anything you have picked up on the tooth pick, there still will be zillions of bacteria on it!
Then grow the bacteria for a day or two, take out a small amount and bubble it with air! Luminescence will ensue! Apparently, luminescence can be so bright that you can read a newspaper with it in the dark!
How cool is that!!


Anyway, apparently it is better to use fresh fish from a fish market next shore lines, simply because transport wasn't so long and the fish are fresh. Water from a salt water aquarium gives varying, but usually poor results. If you live inland and happen to be on a seacoast, place a fresh squid in a plastic bag with trapped air and place it in crushed ice for storage for the trip home. For better results place fish or part of a fish in a jar that is pushed into crushed ice in a cooler. The purpose of the jar is to protect any growing colonies from smearing. By the time you get home glowing colonies may be present. After one or two days at 4 to 18C, luminous colonies may be present. Examine the squid or fish daily. Incubation at 15 to 20C may be the best temperature. Or use your home refrigerator which is about 4 C or above.

By the way, Photobacterium phosphoreum is the strongest light emitting bacterium known!
Whoever tries this gets the Mad Biochemist award! :D


PS An alternative, but similar growth medium is
30 g NaCl
1 g Glycerol
10 g Peptone (Bacto-Peptone)
3 g Beef Extract
(to obtain solid medium you should add 15 g of Bacto-Agar)
Dilute the ingredients in water (pH should be about 7,3)
Fill with H2O up till 1000 ml

PS2 At AEM Publications, I found a paper 'Isolation and Identification of Photobacterium phosphoreum from an Unexpected Niche: Migrating Salmon' by Budsberg et al. they use 0.38 M NaCl, 0.02 M MgCl2 · 6H2O, 0.25 M MgSO4 · 7H2O, 8 mM KCl, 0.5% peptone, 0.3% yeast extract, 0.3% glycerol as a growth medium. The first few are artificial seawater, which by itself is 0.4 M NaCl, 0.1 M MgSO4 · 7H2O, 0.02 M KCl, 0.02 M CaCl2 · 2H2O.

[Edited on 7-2-2004 by chemoleo]


Awesome. they ought to coat the toilets with a triboluminescent zinc layer

View user's profile Visit user's homepage View All Posts By User
12332123
Harmless
*




Posts: 38
Registered: 14-11-2009
Member Is Offline


[*] posted on 15-4-2010 at 07:01


I know this thread is somewhat old, but I just wanted to recount an experiment we did in biology last year (14 yo). We inserted bacterial plasmids engineered to contain antibiotic resistance and a gene from jellyfish which produces a UV fluorescent protein into some bacteria, then killed off the bacteria which had not taken up the plasmid using an antibiotic solution (very clever IMHO). This required a very specific procedure involving cold shock and I didn't get any colonisation :( but it worked for some other groups. I remember my biology teacher saying that the required materials cost several hundred pounds.

[Edited on 15-4-2010 by 12332123]
View user's profile View All Posts By User
The WiZard is In
International Hazard
*****




Posts: 1617
Registered: 3-4-2010
Member Is Offline


[*] posted on 15-4-2010 at 10:27


The most common source of luminescence in da sea
dobe luminous alga e.g., Pyrocystis luna and Pyrocystis noctiluca.

This may take all the fun of self discovery out -

Wards sells a —

Fascinate Students with One of the Simplest Light-Producing Organisms Luminescent Bacteria Living Specimen Set

These glowing green and bluish-green examples of bioluminescence make a fascinating addition to microbiology studies. Cultures include: Azotobacter vinelandii and Vibrio fischeri. The set comes with photobacteria medium, instructions, and the Working with Bacteria manual.

http://wardsci.com/product.asp?pn=IG0007238

If the back of whats left of my mind was --- Difco sold photobacteria medium, however, I find not it listed.

Run Photobactium medium through Google or same such.
View user's profile View All Posts By User
The WiZard is In
International Hazard
*****




Posts: 1617
Registered: 3-4-2010
Member Is Offline


[*] posted on 15-4-2010 at 12:02


Let me introduce a dirty word here — Book.

People on la Net spit and scream when someone suggests
a book and absolutely have sissy-fits if you suggest they
to the (an even dirtier word) Library.

I would mention in passing :—

E Newton Harvey's 692 page opus.

A History of Luminescence : From the Earliest Times Until 1900
American Philosophical Society
Philadelphia 1957

He discusses luminescence in all it variations, e.g.,

The Journal of industrial and Engineering Chemistry
2 [11] 478-9. November, 1910.

Tribo-Luminescence. [1]

The term “luminescence” has been applied to the light which is emitted by various substance at a temperature much lower than that which is necessary to show visible incandescence, as illustrated for example in the light of the firefly, decaying wood, and many natural and artificial mineral compounds.

The general phenomenon of luminescence has been divided into several classes by Dr. Sylvanus P. Thompson, as shown in the following tabulation which is copied from his book entitled "Light Visible and Invisible," page 175 (1893).

Phenomenon. Substance in which it occurs.

1. Chemi-luminescence………... Phosphorus oxidizing in moist air;
decaying wood; decaying fish; glow-worm;
firefly; marine organisms, etc.
2. Photo-luminescence
(a) Transient: Fluorescence............Fluor-spar; uranium-glass; quinine;
scheelite; platino-cyanides of vari-ous
bases; eosin and many coal-tar products.
(b) Persistent: Phosphorescence…. Bologna-stone; Canton's phosphorus
and other sulphides of alkaline earths;
some diamonds; etc.
3. Thermo-luminescence ……………. Fluor-spar; scheelite.
4. Tribo-luminescence……………. Diamonds; sugar, quartz; uranyl
nitrate; pentadecyl-paratolylketone.
5. Electro-luminescence
(a) Effuvio-luminescence Many rarefied gases; many of the
fluorescent and phosphorescent bodies.
(b) Kathodo-luminescence Rubies; glass; diamonds; many gents
and minerals.
6. Crystallo-luminescence………… Arsenious acid.
7. Lyo-luminescence…………… Sub-chlorides of alkali metals.
8. X-luminescence…………….… Platinoeyanides; scheelite; etc.'

Light Visible and Invisible can be DL'd from Google.com/books.

View user's profile View All Posts By User
Sedit
International Hazard
*****




Posts: 1922
Registered: 23-11-2008
Member Is Offline

Mood: Manic Expressive

[*] posted on 15-4-2010 at 12:06


Quote: Originally posted by The WiZard is In  
Let me introduce a dirty word here — Book.

People on la Net spit and scream when someone suggests
a book and absolutely have sissy-fits if you suggest they
to the (an even dirtier word) Library.



Well, Welcome to Science madness where we will spit and scream if your ass don't read a book before speaking.





Knowledge is useless to useless people...

"I see a lot of patterns in our behavior as a nation that parallel a lot of other historical processes. The fall of Rome, the fall of Germany — the fall of the ruling country, the people who think they can do whatever they want without anybody else's consent. I've seen this story before."~Maynard James Keenan
View user's profile View All Posts By User
peach
Bon Vivant
*****




Posts: 1428
Registered: 14-11-2008
Member Is Offline


[*] posted on 6-10-2010 at 10:07


Not a bacteria, but here's Mycena luxaeterna from the mushroom kingdom doing it out in the wild.





And Mycena chlorophos.







View user's profile View All Posts By User
ScienceSquirrel
Super Moderator
Thread Pruned
13-3-2012 at 05:36
 Pages:  1  2  

  Go To Top