| Pages:
1
2
3 |
Trifluoroacetic
Hazard to Others
Posts: 128
Registered: 6-8-2008
Member Is Offline
|
|
DNA Extraction and Electrophoresis
Hi guys I've been on here before under a different name but it's been a while and I forgot my user ID and password.
Anyway I thought I would let you know that I have been doing some DNA extractions. I am in the process of experimenting with running the DNA through
an electrophoresis unit and staining the DNA bands with various Dyes.
I made my own gel tracking dyes and buffers and am using Agar ager from a food mart.
I do have a company that has sent me a bunch of free supplies and I do have the ability to order real enzymes, ethidium bromide, buffers, and agarose;
but at this time I am using the supplies I have on hand.
right now I am experimenting with staining DNA with Nile Blue and Methylene blue Chloride. I have a bunch of pictures if anyone wants me to post them.
Just let me know.
|
|
|
Trifluoroacetic
Hazard to Others
Posts: 128
Registered: 6-8-2008
Member Is Offline
|
|
DNAExtraction
|
|
|
Trifluoroacetic
Hazard to Others
Posts: 128
Registered: 6-8-2008
Member Is Offline
|
|
DNAExtraction
Here is a test run of my homemade tracking Dye.
|
|
|
mac
Harmless
Posts: 1
Registered: 12-11-2008
Member Is Offline
|
|
Hi Trifluoracetic,
This is my first time at the sciencemadness forums - they seem great! I just wanted to let you know that some friends and I are trying to develop and
publicize (via instructables and videos at diybio.org) a DIY gelbox that is pro-grade and includes a transilluminator (blue LEDs).
We posted a fun DNA extraction instructable a week ago, and when we get the gel box working we'll post some more explaining how to build one and how
to extract and purify DNA to run through it. http://www.instructables.com/id/5_minute_DNA_Extraction_in_a...
Want to help? Ping me: mac at diybio.org
|
|
|
Trifluoroacetic
Hazard to Others
Posts: 128
Registered: 6-8-2008
Member Is Offline
|
|
I've checked out the website it's very cool keep me updated. When I get time to do more experimenting I will do the same.
|
|
|
Trifluoroacetic
Hazard to Others
Posts: 128
Registered: 6-8-2008
Member Is Offline
|
|
I'd be more then happy to help out with the project.
|
|
|
chief
International Hazard
Posts: 630
Registered: 19-7-2007
Member Is Offline
|
|
DNA-extraction for everyone, very good idea. So one could track all the litle crimes, which happen and are not provable (Who stole the letter from the
postbox ? Who read my private documents? Who sabotaged this or that ? etc.) oneself.
Or: Who let's his dog shit in front of my door all the time ?
Can it be done without too special or expensive chemicals ? I may order a lot of different things, too ... but neither want to get ripped for the
money nor appear in the wrong context in any databases ... ?
Besides: All those private detectives everywhere: One could establish a service for them; ... it would help a lot definately ... !
It definately should not be regulated by any law, since it's probably only lightweight chemistry and a bit of electrophoresis ... so it would be legal
to offer it as a service !
At least the gel-column could be made oneself, then be adjusted via a known standard-substance (2 runs: one with standard, one without; the the
scaling via the standard would be possible) ...
[Edited on 10-12-2008 by chief]
[Edited on 10-12-2008 by chief]
|
|
|
Trifluoroacetic
Hazard to Others
Posts: 128
Registered: 6-8-2008
Member Is Offline
|
|
DNA is analyzed through electrophoresis visually by comparing the bands to molecular weight markers that are added to the first column in the gel.
It really that hard to extract DNA and purify it or even do electrophoresis with it. The chemicals that are required in most cases are easily obtained
and quite cheap.
The only real problem that I can see with conducting an Actual DNA analysis is with obtaining and storing the restriction enzymes and PCR enzymes.
They have to be stored at a -20 degrees and are quite expensive. But with that in mind I'd still like to buy some and do some real experimenting.
|
|
|
| Pages:
1
2
3 |
![[*]](images/xpblue/default_icon.gif){kind=icon}
