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Author: Subject: Agar substitutes
White Yeti
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[*] posted on 9-4-2012 at 05:19


Definitely, go right ahead! Be sure to post a procedure on how you did it.

I am currently growing a liquid culture of Trametes versicolor. After that, I'm going to have to use PF Tek, a technique I've never tried before to get the fungus to colonise a slab of growth medium. I was planning on modifying the technique a bit by directly injecting mycellium into the jar instead of messing around with spore syringes and what not. In any case, this fungus does not propagate nearly as quickly as the penicillium (which is to be expected). I won't be 100% sure I have the right fungus until it starts to fruit. All mycelia look alike, white and fuzzy.




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Eddygp
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[*] posted on 14-4-2012 at 09:29
My LC Fungi


Surprising results... From a small patch of fungi, I have now dozens of little mycelium dots scattered all over the LC, apart from the two larger blobs.
Add some green food colouring to tap water. Add 5 drops of vinegar and 2 drops of diluted starch. Add sugar in an aqueous solution (as much as you want). Inoculate some spores.
I'm going to cultivate some macroscopic fungi, probably Fomes, Trametes or Grifola species, but I will do first some research on spore syringes.

[Edited on 14-4-2012 by Eddygp]

fungi 004.jpg - 301kB

[Edited on 14-4-2012 by Eddygp]




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[*] posted on 15-4-2012 at 19:39


Hi Eddygp,

This looks very nice and is likely a great media for growing moulds, many of which are oligotrophic and will not require many nutrients (I've seen some grow on nothing more than molecular grade agarose and ultrapure water). But for growing larger species like you are hoping to try it likely won't work since these are going to require large amounts of nutrients other than fermentable carbon. A good complex media that may work and is very easy to make is PDA (Potato Dextrose Agar) http://en.wikipedia.org/wiki/Potato_dextrose_agar this can be used as a broth as well since you don't have agar, and sucrose can replace the dextrose. The potatoes used in making this media will provide all the other nutrients needed.

Good luck with your fungi cultivation and keep us posted!
-ssdd




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[*] posted on 16-4-2012 at 00:50


Thank you, ssdd!
I have been thinking of growing saprotrophic fungi, and will probably use that agar. However, I have also thought of growing them on wood. I have read that many saprotrophic fungi prefer mildly acidic conditions (which are quite easy to make, adding a bit of vinegar). Thank you for the idea!

Eddy

PS. Would flour be useful to subtitute the agar?

[Edited on 16-4-2012 by Eddygp]




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[*] posted on 16-4-2012 at 13:10


@Eddygp, sorry for the late reply, but that culture looks fantastic! I like your idea to use food colouring to show the fungus better. I didn't try adding vinegar either, but this is definitely something I'll be trying soon. I think I'll try to grow Agaricus bisporus (Portobello mushroom) with the PF-tek technique instead of Trametes versicolor because it seems like my culture failed:( Either I contaminated the plate, or another fungus is feeding off of the fungus I introduced into the growth medium. I don't know for sure, so that's why I'm starting over.



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[*] posted on 17-4-2012 at 14:37


Dunno if flour would work, it might do OK, but I could see it being a real pain to sterilize to any level (unless you have a pressure cooker or something).

Something else that might work (just throwing some untested ideas out here) is using a solid substrate like pearlite and soaking liquid media onto them. But then pearlite would also be difficult to sterilize...

Let us know what you find that works!

I do know of a few exoctic, but probably hard to get, agar substitutes: Gellan Gum (works nicely, but needs +2 ions to solidify, once solid you can't really melt it again), Polyvinyl Alcohol (makes a nice think syrup), Gelatin of course but its easily broken down, etc...

-ssdd




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[*] posted on 20-4-2012 at 02:09


My fungi are growing very quickly!



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[*] posted on 1-5-2012 at 12:58


Anyone still interested in this topic?



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[*] posted on 1-5-2012 at 14:58


Yes. Progress is slow and rather uneventful, yet ongoing. I'm working on a growth medium from a corn starch, egg whites, sucrose, food colouring, and various micronutrients (iron, zinc, copper). Dosing micronutrients is very important, too much iron and nothing grows. I'll also add a little vinegar to lower the pH.

At the moment I'm swamped, I'm preparing for four AP exams, no free time whatsoever.




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[*] posted on 3-5-2012 at 13:05


Good luck! My fungi withered away by a factor I still do not understand... I'll see if this autumn I get some small saprotrophic fungi to grow on a sucrose-flour-sawdust mixture I'm planning.



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[*] posted on 5-6-2012 at 10:01


I'm leaving this for a while. In October or so, I will try to get some small err... saprotrophic fungi to cultivate from spores.



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[*] posted on 19-11-2012 at 08:57


did you manage to do anything eddygp?
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[*] posted on 23-11-2012 at 13:19


Actually, I did. But not exactly what I was intending to do... -.-
In a nutshell...
I tested many fungicides and antibiotics on some fungi cultures I had, as well as bacteria. I also compared them wit ha slice of freshly cut garlic. Umm, the fungicides worked just as I expected, with low concentration being very harmful. The antibiotics I used only left some strange boneish white bacteria colonies (very very small). The garlic slice had made a circle with radius ~ 0.9 cm with no bacteria or fungi. However, some very interesting orange colonies of bacteria had grown... on top of the garlic!!! These bacteria have developed some sort of resistance to the substances garlic has, and they seem to grow only there. I guess garlic should some day be decomposed or the world would be full of garlic slices... :cool:
Yeah, just about that, without speaking of the crickets (Toby, Sophie, August and Tora (long story)) or the fungi identification (the biology dep. didn't have any species for its collection, they only had some shrews, frogs, etc.).




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[*] posted on 23-11-2012 at 22:58


Anyone try polyacrylamide crystals? I've doped them with a little miracle grow for plant growth medium experiments, and had fungus outbreaks on them, so I had to add a small amount of con-san to the mix to prevent it. biodegrades into ammonia and nitrogen gas.



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[*] posted on 26-11-2012 at 10:55


Hmm I haven't tried those.



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[*] posted on 9-12-2012 at 12:52


I have made a spore print of an Inocybe species



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