Sciencemadness Discussion Board
Not logged in [Login ]
Go To Bottom

Printable Version  
Author: Subject: Gelatin for gradient centrifuging?
magnus454
Hazard to Self
**




Posts: 57
Registered: 28-2-2007
Location: Clear Lake City, TX
Member Is Offline

Mood: No Mood

[*] posted on 4-10-2011 at 20:29
Gelatin for gradient centrifuging?


Has anybody used plain gelatin or agar to make a gradient for centrifuging? If so what formula did you use to get the different densities?



History is repeating itself.
View user's profile Visit user's homepage View All Posts By User
phlogiston
International Hazard
*****




Posts: 1344
Registered: 26-4-2008
Location: Neon Thorium Erbium Lanthanum Neodymium Sulphur
Member Is Offline

Mood: pyrophoric

[*] posted on 21-10-2011 at 16:01


What do you want to do with it, exactly? Probably, you want to make a density gradient, but it doesn't seem very suitable for that. You would need to dissolve a lot to get any relevant density increase, and then the holes in the maze are going to be too small for larger biomolecules or organelles to pass through.

If you something cheap and readily available, use sucrose (common table sugar). I've separated liposomes and organelles with it with good success.





-----
"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer
View user's profile View All Posts By User
anonymous201
Harmless
*




Posts: 31
Registered: 21-10-2011
Location: Spain
Member Is Offline

Mood: No Mood

[*] posted on 21-10-2011 at 16:16


More details:)
View user's profile View All Posts By User

  Go To Top