magnus454
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Gelatin for gradient centrifuging?
Has anybody used plain gelatin or agar to make a gradient for centrifuging? If so what formula did you use to get the different densities?
History is repeating itself.
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phlogiston
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What do you want to do with it, exactly? Probably, you want to make a density gradient, but it doesn't seem very suitable for that. You would need to
dissolve a lot to get any relevant density increase, and then the holes in the maze are going to be too small for larger biomolecules or organelles to
pass through.
If you something cheap and readily available, use sucrose (common table sugar). I've separated liposomes and organelles with it with good success.
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"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer |
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anonymous201
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