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Author: Subject: Extracting the alkaloid scopolamine from datura stramonium seeds?
Mike1.618
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[*] posted on 28-7-2006 at 02:24
Extracting the alkaloid scopolamine from datura stramonium seeds?


does anyone have a more effective technique for extracting the alkaloid scopolamine from datura seeds than the following, and can anyone confirm the validity of the following technique?

STEP #1 Grind 2 grams dantra seed in mortar and pestle.
STEP #2 Place seed pulp into test tube.
STEP #3 Use pipette to place 6mL methyl alcohol into test tube with seed pulp. (the entire contents of the test tube should be 9mL this includes alcohol and seed pulp.)
STEP #4 Shake very well.
Note 1: methanol soon turns a clear yellowish color.
Note 2: end of day one there was only a slight darkening of the alcohol.
Note 3: end of day two there was no change in the appearance of the methanol.
STEP #5 Filter methanol from seed pulp. (gravity filtration will work fine for this).
STEP #6 Pour 2 mL. methanol through seed pulp to wash any remaining alkaloids from the seeds.

NOTE: Upon evaporation of methanol you are left with a golden yellow crystalline powder. The total product of this extraction is 80 mg.
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Ramiel
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[*] posted on 28-7-2006 at 05:04


No triple posting, you're skating on thin ice Mike. Besides, organic exctractions are pretty basic organic chemistry. If you really aren't sure what would work or why, I think you should READ some good texts before you DO something that may HURT someone.



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Vitus_Verdegast
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[*] posted on 28-7-2006 at 09:00


Stay away from scopolamine. You'll probably end up in the hospital yourself if you attempt to pull this one off.



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DDTea
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[*] posted on 28-7-2006 at 09:25


Don't scare him; atropine and scopolamine aren't so bad. Just wear some decent gloves. But judging by this guy's post on totse [exactly the same] and his twisted intentions...you never know.



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[*] posted on 28-7-2006 at 09:31


Do take the advice given meanwhile you can read about it ...............solo.

---------------------------------------------------------------------------------------------

Alkaloid Production in Diploid and Autotetraploid Plants of Datura stramonium
Strahil Berkov1 and Stefan Philipov2
Pharmaceutical Biology 2002, Vol. 40, No. 8, pp. 617–621

Abstract
A comparative investigation of alkaloid production and accumulation in the roots and leaves of diploid (2n = 24), and C4 generation of induced autotetraploid (4n = 48) Datura stramonium L. plants was performed. Fifteen alkaloids have been determined in the roots and two in the leaves, at a level of 1% or more of the crude alkaloid fractions in both ploidy levels. Two 3-tigloyloxy-6-isovaleryloxy-7-
hydroxytropane isomers were detected for the first time in genus Datura. In the conditions of the prolonged photoperiod, hyoscyamine was the main alkaloid in the roots whereas scopolamine was the main alkaloid in the leaves of both ploid
forms. In comparison to diploids, the roots and leaves of tetraploids had a higher alkaloid content and scopolamine/ hyoscyamine ratio.

Keywords: Datura stramonium, induced polyploidy, tropane
alkaloids.


Excerp
....Alkaloid extraction:

Root and leaf samples (0.2–0.5 g) were ground up with sea
sand and macerated in 10ml 3% H2SO4 for 6 h at room temperature.
After filtering and washing of the plant residue with
5 ml of distilled water, the solutions were adjusted to pH 9–10
with 25% NH4OH and triple extracted with 10ml CHCl3. The
combined chloroform extracts were dried over anhydrous
Na2SO4, filtered and evaporated in vacuo to give the crude
alkaloid fractions. Thus obtained residues were resolved in
CH3OH for further analysis.

Attachment: Alkaloid Production in Diploid and Autotetraploid Plants of Datura stramonium .pdf (52kB)
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Vitus_Verdegast
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[*] posted on 28-7-2006 at 09:37


I know, but as you say it smells very much like totse...
Although I am all for Darwinism, I'm too weak-hearted to endure the vision of Mike1.618 becoming Mike]-∞[ by spilling the methanol containing the anticholinergic deliriants all over his little hands. ;)




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DDTea
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[*] posted on 28-7-2006 at 09:53


By the way, if you have your hands on some Datura seeds, I'd strongly recommend growing some! It's very uplifting and fun. I love my little datura plants, and I have one big one, maybe 8" high with very pretty/weirdish looking leaves. I can't wait for them to start flowering. Daturas are beautiful plants! [and you will get better alkaloid yields from fresh plants] :)



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[*] posted on 28-7-2006 at 10:17


I know, I know!:):) I'm a big fan myself.

For the moment I don't have place to grow these plants, but I can comfort myself with the 6 year old wild Atropa belladonna that is growing not far from my place. :D A stunningly beautiful plant!

Every year I go collecting seeds from above belladona and every Datura stramonium that comes on my way.

When I do have access to a garden again, I plan to get ahold of some Hyoscyamus, Mandragora and Scopolia species. Also a friend of mine has an Atropa acuminata from the Himalayas.




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