Mike1.618
Harmless
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Registered: 28-7-2006
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Extracting the alkaloid scopolamine from datura stramonium seeds?
does anyone have a more effective technique for extracting the alkaloid scopolamine from datura seeds than the following, and can anyone confirm the
validity of the following technique?
STEP #1 Grind 2 grams dantra seed in mortar and pestle.
STEP #2 Place seed pulp into test tube.
STEP #3 Use pipette to place 6mL methyl alcohol into test tube with seed pulp. (the entire contents of the test tube should be 9mL this includes
alcohol and seed pulp.)
STEP #4 Shake very well.
Note 1: methanol soon turns a clear yellowish color.
Note 2: end of day one there was only a slight darkening of the alcohol.
Note 3: end of day two there was no change in the appearance of the methanol.
STEP #5 Filter methanol from seed pulp. (gravity filtration will work fine for this).
STEP #6 Pour 2 mL. methanol through seed pulp to wash any remaining alkaloids from the seeds.
NOTE: Upon evaporation of methanol you are left with a golden yellow crystalline powder. The total product of this extraction is 80 mg.
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Ramiel
Vicious like a ferret
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No triple posting, you're skating on thin ice Mike. Besides, organic exctractions are pretty basic organic chemistry. If you really aren't sure what
would work or why, I think you should READ some good texts before you DO something that may HURT someone.
Caveat Orator
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Vitus_Verdegast
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Stay away from scopolamine. You'll probably end up in the hospital yourself if you attempt to pull this one off.
Sic transit gloria mundi
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DDTea
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Don't scare him; atropine and scopolamine aren't so bad. Just wear some decent gloves. But judging by this guy's post on totse [exactly the same]
and his twisted intentions...you never know.
"In the end the proud scientist or philosopher who cannot be bothered to make his thought accessible has no choice but to retire to the heights in
which dwell the Great Misunderstood and the Great Ignored, there to rail in Olympic superiority at the folly of mankind." - Reginald Kapp.
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solo
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Do take the advice given meanwhile you can read about it ...............solo.
---------------------------------------------------------------------------------------------
Alkaloid Production in Diploid and Autotetraploid Plants of Datura stramonium
Strahil Berkov1 and Stefan Philipov2
Pharmaceutical Biology 2002, Vol. 40, No. 8, pp. 617–621
Abstract
A comparative investigation of alkaloid production and accumulation in the roots and leaves of diploid (2n = 24), and C4 generation of induced
autotetraploid (4n = 48) Datura stramonium L. plants was performed. Fifteen alkaloids have been determined in the roots and two in the leaves, at a
level of 1% or more of the crude alkaloid fractions in both ploidy levels. Two 3-tigloyloxy-6-isovaleryloxy-7-
hydroxytropane isomers were detected for the first time in genus Datura. In the conditions of the prolonged photoperiod, hyoscyamine was the main
alkaloid in the roots whereas scopolamine was the main alkaloid in the leaves of both ploid
forms. In comparison to diploids, the roots and leaves of tetraploids had a higher alkaloid content and scopolamine/ hyoscyamine ratio.
Keywords: Datura stramonium, induced polyploidy, tropane
alkaloids.
Excerp
....Alkaloid extraction:
Root and leaf samples (0.2–0.5 g) were ground up with sea
sand and macerated in 10ml 3% H2SO4 for 6 h at room temperature.
After filtering and washing of the plant residue with
5 ml of distilled water, the solutions were adjusted to pH 9–10
with 25% NH4OH and triple extracted with 10ml CHCl3. The
combined chloroform extracts were dried over anhydrous
Na2SO4, filtered and evaporated in vacuo to give the crude
alkaloid fractions. Thus obtained residues were resolved in
CH3OH for further analysis.
Attachment: Alkaloid Production in Diploid and Autotetraploid Plants of Datura stramonium .pdf (52kB)
This file has been downloaded 3666 times
It's better to die on your feet, than live on your knees....Emiliano Zapata.
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Vitus_Verdegast
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I know, but as you say it smells very much like totse...
Although I am all for Darwinism, I'm too weak-hearted to endure the vision of Mike1.618 becoming Mike]-∞[ by spilling the methanol containing
the anticholinergic deliriants all over his little hands. 
Sic transit gloria mundi
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DDTea
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By the way, if you have your hands on some Datura seeds, I'd strongly recommend growing some! It's very uplifting and fun. I love my little datura
plants, and I have one big one, maybe 8" high with very pretty/weirdish looking leaves. I can't wait for them to start flowering. Daturas are
beautiful plants! [and you will get better alkaloid yields from fresh plants] 
"In the end the proud scientist or philosopher who cannot be bothered to make his thought accessible has no choice but to retire to the heights in
which dwell the Great Misunderstood and the Great Ignored, there to rail in Olympic superiority at the folly of mankind." - Reginald Kapp.
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Vitus_Verdegast
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Location: Ottoman Empire
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I know, I know! I'm a big fan myself.
For the moment I don't have place to grow these plants, but I can comfort myself with the 6 year old wild Atropa belladonna that is growing not far
from my place.  A stunningly beautiful plant!
Every year I go collecting seeds from above belladona and every Datura stramonium that comes on my way.
When I do have access to a garden again, I plan to get ahold of some Hyoscyamus, Mandragora and Scopolia species. Also a friend of mine has an Atropa
acuminata from the Himalayas.
Sic transit gloria mundi
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