Little_Ghost_again
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Diatamaceous earth
Hi,
Does anyone know or has anyone used diatomaceous earth for the solid phase in liquid chromatography?
Silicon gel one has jumped in price lately, I am trying to isolate samples from different plant extracts.
Cheers
Dont ask me, I only know enough to be dangerous
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byko3y
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Buy a usual silica gel (beads), crush it with hammer, sift through a large sieve to remove really big particles. Obtained silica gel will have a
variable particle size, but is sufficient for simple chromatography.
[Edited on 26-9-2015 by byko3y]
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battoussai114
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It's used in GC from what I've heard. If you bother doing a little acid washing to ensure you only have Silica on your diatomaceous earth I'd guess
you'd be fine.
Batoussai.
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Little_Ghost_again
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Quote: Originally posted by battoussai114  | | It's used in GC from what I've heard. If you bother doing a little acid washing to ensure you only have Silica on your diatomaceous earth I'd guess
you'd be fine. |
I will give that a go, silica seems to have gone to silly prices. I guess I could wash it and ball mill a bit. The stuff I was using is now £22 for
500g, seems steep when you get 5-6 columns out of it,
The cheaper stuff varies widely in particle size and hasnt given good separation. I did think of reusing the gell but everything I have seen and read
advises against this
Dont ask me, I only know enough to be dangerous
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Little_Ghost_again
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| Quote: Originally posted by byko3y | Buy a usual silica gel (beads), crush it with hammer, sift through a large sieve to remove really big particles. Obtained silica gel will have a
variable particle size, but is sufficient for simple chromatography.
[Edited on 26-9-2015 by byko3y] |
this one
http://www.ebay.co.uk/itm/8-KG-Silica-Gel-for-column-chromat...
is mesh 70-230 and £120 for 8Kg, so the commercial stuff varies widely in size anyway, if I could find the beads at a reasonable price I would ball
mill them, I dont get why its jumped so high in price over the last 4 months
Dont ask me, I only know enough to be dangerous
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