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Author: Subject: Putrescine Toxicity
Agari
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[*] posted on 29-10-2015 at 22:36
Putrescine Toxicity


I have stumbled upon a substance called Putrescine,C4H12N2,which is a foul-smelling substance,yet was not toxic at first glance. However,after reading about it on Wikipedia, it was stated that putrescine has a lethal dose of 463 mg/kg of body weight when ingested and 1 gram per kilogram when absorbed dermally. My question is: How will putrescine kill me?Is it metabolised to toxic substances (Like methanol is metabolised to formaldehyde and formic acid),is it neurotoxic,a blistering agent, or does it cause damage in the same manner as hydrofluoric acid?

[Edited on 30-10-2015 by Agari]
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Amos
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[*] posted on 30-10-2015 at 03:43


That's around the same LD50 as aspirin; do you really consider that toxic? Here's quite a large amount of information, but it's not nearly so exotic as a blistering agent; we are talking about a substance naturally occurring in humans

http://toxnet.nlm.nih.gov/cgi-bin/sis/search/a?dbs+hsdb:@ter...
http://www.researchgate.net/publication/228083797_Dietary_ex...

From a quick scan, I found that putrescine is a potentiator of other amines in the body, notably histamine, meaning it strengthens the effects of that and other amines. It is also broken down in the liver to an extent, meaning it may be hepatotoxic. As for symptoms:
"Symptoms that can occur after excessive oral intake of biogenic amines include nausea, respiratory distress, hot flush, sweating, heart palpitations, headache, bright red rash, burning sensations in the mouth and alterations in blood pressure"




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kecskesajt
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[*] posted on 30-10-2015 at 08:49


The smell will kill you first.Before it kill you there is a high chance that you will run out of the lab.
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Dr.Bob
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[*] posted on 30-10-2015 at 10:30


It acts as a weak cationic lipid, which is a membrane disruptor, not much different than eating Lysol or detergent. Much like dialkylbenzylmethylammonium salts acts as detergents, polyamines can change the behavoirs of your cell's membrane, which can be bad for them. But it take a lot, much like ethanol can alter your cells membrane, along with other receptors, which is large doses can be fatal. But the smell would cause to to vomit yourself to death long before you could inject 46 grams of it.
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hissingnoise
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[*] posted on 30-10-2015 at 10:32


Yeah, it's hard to eat something if the smell makes you want to throw up?

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[*] posted on 30-10-2015 at 11:54


My guess is that its toxicity is based on the fact, that if you rub yourself down with it, possums will eat you while you are sleeping.
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Agari
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[*] posted on 30-10-2015 at 13:52


What is with all of the joke replies such as "The smell will kill you"?

Big shoutout to ""Dr.Bob" and Amos.

"That's around the same LD50 as aspirin; do you really consider that toxic? Here's quite a large amount of information, but it's not nearly so exotic as a blistering agent; we are talking about a substance naturally occurring in humans

http://toxnet.nlm.nih.gov/cgi-bin/sis/search/a?dbs+hsdb:@ter...
http://www.researchgate.net/publication/228083797_Dietary_ex...

From a quick scan, I found that putrescine is a potentiator of other amines in the body, notably histamine, meaning it strengthens the effects of that and other amines. It is also broken down in the liver to an extent, meaning it may be hepatotoxic. As for symptoms:
"Symptoms that can occur after excessive oral intake of biogenic amines include nausea, respiratory distress, hot flush, sweating, heart palpitations, headache, bright red rash, burning sensations in the mouth and alterations in blood pressure"
That does actually sound like it would be an effect of histamine response.
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[*] posted on 30-10-2015 at 16:39


I have worked with 1,4-diaminobutane (putrescine) in the past, I did not find out the trivial name of the compound until after I had worked with it a few times. I did not find the smell any more unsettling than any of the diamines around it (of course cadaverine is only one carbon longer). Sure it smells but I would hardly call it horrible.



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[*] posted on 31-10-2015 at 11:56


"potentiator of other amines" seems to be a fairly good explanation on what it does.

I would say that based on the description of symptomes of poisoning putrescine is toxic mostly because it saturates the monoaminoxidases in the body. While the enzymes are busy destroying the compound they "have no time" to do the same with the otherwise (much) more toxic endogenous (and food-based) substances e.g. hystamine, adrenaline, noradrenaline, tyramine, etc.
These sympathomimetic amines build up in the body and cause the symptomes described.

In high doses it may have direct membrane-desintegrating properties as well.
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Agari
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[*] posted on 2-11-2015 at 14:40


Quote: Originally posted by Pumukli  

In high doses it may have direct membrane-desintegrating properties as well.

So, it can be used as a lysis buffer should I get bored and do the classical banana DNA extraction experiment?
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[*] posted on 3-11-2015 at 00:19


I don't know the classical banana experiment because we extracted DNA from E. coli bacteria. We used Na-laurylsulphate to disintegrate the cells if memory serves well.
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Agari
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[*] posted on 3-11-2015 at 14:35


Quote: Originally posted by Pumukli  
I don't know the classical banana experiment because we extracted DNA from E. coli bacteria. We used Na-laurylsulphate to disintegrate the cells if memory serves well.

Since a lot of amateurs stumble across DNA extraction yet don't have access to an adequate amount of bacteria or lytic agents,they would use detergents and the such to cause lysis and extract the DNA from plants instead. A good example would be the video by recently-deceased SM member MyFanwy(I had to mention him,it tends to pull some emotional strings around here).I only wanted to make putriscene because of its strong odor,not to use as a lysis buffer,but the fact that it can disintegrate cellular membranes would suggest that it can be used as such.



[Edited on 3-11-2015 by Agari]
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