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Author: Subject: mass spectrum of tritium labelled peptides?

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Registered: 3-5-2018
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[*] posted on 3-5-2018 at 23:49
mass spectrum of tritium labelled peptides?

Hi all,

I have some questions regarding tritium labelled peptides, some uncertainties I have and would greatly help me if I could talk with somebody with more knowledge in the field of tritium and mass spctrometry. One of my main questions is why is never reported a mass of a tritium labelled peptide? Is it actually possible? For C14 I was able to measure the mass, but not 3H... I do consider the high specificity of eg. 3H in comparation with 14C. One more thing, if one calculates 1 mCi of 3H with 60 Ci/mmol gives an amount of 16.6 nmol...for HPLC, of course one takes a small sample so one gets in the pico area... how is able one to still have an UV peak in the HPLC?

Please help.

I did not find the right solution from the internet.

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[*] posted on 4-5-2018 at 00:49

Ditto the other three threads.
Nice try but no spamming here.

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A primer on metals and non-metals with at least one novel experiment.
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