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Author: Subject: Sequencing DNA
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[*] posted on 8-9-2007 at 07:44
Sequencing DNA


Can someone tell me how to sequence DNA?



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[*] posted on 8-9-2007 at 12:50


Nowadays you send it out, or have your undergrads run those hideous gels for you. =]
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[*] posted on 14-9-2007 at 00:01


your target sequence should be no more than 1000 base pairs long. First you aneal your oligonucleotide primers to each end of your single stranded DNA. Then using taq, radioactive phosporus and dT dC dA dG you fill in varying lengths of your sequence and finally run that on an acrylamide gel and read the autoradiograph sequence you get after putting some film on the gel for 12 hours in the -80 freezer

piece of cake!

Or you can just send your sample off to the core and let them do it! :P

[Edited on 14-9-2007 by Maya]




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