Karl Fischer titration

Karl-Fischer titration, often shortened to KF titration, is an iodometric titration technique for the determination of water content in a sample. The reagents contain, as a minimum, elemental [[iodine], sulfur dioxide, and a suitable base.

The overall reaction which occurs is:

2H₂O + SO₂ + I₂ ⇌ H₂SO₄ + 2HI

Base

A base must be present in order to drive the equilibrium to the right by reacting with the sulfuric acid and hydriodic acid produced. Fischer's original reagent used pyridine, however modern KF reagents tend to use other bases like imidazole or pyridine derivatives, due to the volatility, toxicity, and unpleasant odour of pyridine.

Solvent

Normally, the reagent components are dissolved in a primary alcohol such as methanol, ethanol, or methyl cellosolve (ethylene glycol monomethyl ether). In these cases, sulfur dioxide reacts with the alcohol to produce the corresponding alkyl sulfite, which is an intermediate in the reaction.

Non-alcoholic KF reagents are also possible, however the reaction mechanism and stoichiometry changes, and the overall system becomes much more sensitive to sample composition. For this reason non-alcoholic KF reagents are seldom used.

Errors in the original Fischer paper

Fischer's 1935 paper discussed pyridine adducts being key to the reaction, and assumes that the methanol used served only as a solvent. The equation presented in his paper was:

2H₂O + SO₂.2Py + I₂ + 2Py → H₂SO₄.2Py + 2(HI.Py)

where Py = pyridine = C₅H₅N.

The left hand side of this equation gives the ratio H₂O : I₂ : SO₂ : Pyridine = 2 : 1 : 1 : 4. However, this was later determined by Smith, Bryant, and Mitchell to be an incorrect ratio in non-aqueous solvents. They determined the ratio was H₂O : I₂ : SO₂ : Pyridine : Methanol = 1 : 1 : 1 : 3 : 1. This gives the following equations:

H₂O + SO₂ + I₂ + 3Py → SO₃.Py + 2(HI.Py)

SO₃.Py + MeOH → Py.HSO₄Me

Verhoef and Barendrecht later determined that the above equations were incorrect, and that the reaction actually proceeds via monomethyl sulfite ion produced by the reaction of SO₂ and methanol:

2 CH₃OH + SO₂ ⇌ CH₃OH₂⁺ + SO₃CH₃^-

They also determined that pyridine's only role in the reaction is as a buffering agent. Therefore, pyridine can be replaced by other organic bases (denoted RN). Adding the base shifts the equilibrium of the above equation to the right:

CH₃OH + SO₂ + RN → [RNH]SO₃CH₃

The KF titration reaction can then be formulated as:

H₂O + I₂ + [RNH]⁺ SO₃CH₃^- + 2 RN → [RNH]⁺ SO₄CH₃^- + 2 [RNH]⁺ I^-

Notice that this equation follows the 1:1:1:3:1 ratio determined empirically by Smith, Bryant, and Mitchell.