Sciencemadness Discussion Board

The Kastle-Meyer test -- Reducing Phenolphthalein

ScienceGeek - 18-3-2008 at 11:49

The Kastle-Meyer test is a test used in forensics to detect blood, whereupon blood is swabbed, the Phenolphthalein solution is added, followed by the addition of Hydrogen Peroxide. The heme- group in blood catalyses the decomposition of H.P.

The clue of this test is that the Phenolphthalein used is not of the "normal" kind, but has been pre- dissolved in alkaline solution, and reduced to a colorless species using zinc powder.

Look here for more information.

My question:
Has anyone got any experience in doing this? I tried it once, but my results were not any good. Is there any other way of reducing Phenolphthalein to its colorless species?
Could not find any information elsewhere...

Thanks

WizardX - 18-3-2008 at 15:44

http://www.ncsu.edu/kenanfellows/2002/pligon/forensics/labs/...
*Luminol: 1.0 g luminal + .5 g Na2CO3 dissolved in 100 ml of distilled water, then add 0.7 g sodium perborate or 0.9 g potassium iodate.
** Kastle- Meyer reagent - This is a reduced phenolphthalein solution. KOH, phenolphthalaein and Zinc. After dissolved add ethanol to 500 ml store in a brown bottle in the refrigerator.

http://www.blogit.dodgy-at-best.com/forensicallyyours/2005/1...

http://www.nzic.org.nz/ChemProcesses/biotech/12A.pdf
http://www.chymist.com/BLOODSTAIN%20PATTERNS.pdf
http://www.mckinneylabs.com/Forensic/notes/Forensic%20Serolo...
http://www.scenesafe.co.uk/pdfs/K160-%20MSDS.pdf
http://www.tfachicago.net/physicalscience/index.php?act=down...

ScienceGeek - 18-3-2008 at 16:42

Wow!
Thank you extremely much, WizardX! :)