andre178 - 2-6-2010 at 10:15
Hello, beginner question, a patent synthesis calls for 4.5 equivalents of Fremy's salt to add to dihydroethidium (60 uM) in 0.1 M phosphate buffer
under stirring.
Can someone ellucidate what the 4.5 equivalents refers to, I'm not sure, is it potassium to the rest of the salt or is this something else?
Ozone - 2-6-2010 at 11:49
I assume equivalent to nitrosyl radical? It's use can be seen here:
http://www.orgsyn.org/orgsyn/orgsyn/prepContent.asp?prep=cv6...
The mechanistic similarity with superoxide is given in the attached paper.
An interesting comparison with TEMPO (et al.) is given in the papers referring to DPPH and roasted coffee, which are also attached.
Cheers,
O3
Attachment: Zielonka et al 2005_Fremys salt Mechanism.pdf (378kB)
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Attachment: Ionita 2003 DPPH fremys tempo.pdf (156kB)
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Attachment: Cammerer and Kroh 2006 antioxidants in coffee TEMPO etc.pdf (349kB)
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DJF90 - 2-6-2010 at 13:45
4.5 eq. of Fremy's salt means use 4.5 moles of it per mole of substrate.