Sciencemadness Discussion Board

Cellulase enzyme

reactofurnace - 1-7-2019 at 19:29

Im trying to convert cellulose for biomass to ethanol. But first i need to convert the cellulose into a usable carbohydrate such as glucose or starch.

Does anyone know how to extract cellulase enzyme? the source I was thinking of were termites.

What y'all think?

Any other possible sources?


BromicAcid - 1-7-2019 at 19:48

Bought mine on eBay in an attempt to dissolve the leaves in my yard.

Metacelsus - 1-7-2019 at 23:05

Quote: Originally posted by reactofurnace  

Does anyone know how to extract cellulase enzyme? the source I was thinking of were termites.

What y'all think?

Any other possible sources?



Expressing it from a transgene in bacteria is probably the best way to go. There's quite a bit of literature on this.

Depending on the biomass, lignin may be an issue.

[Edited on 2019-7-2 by Metacelsus]

UC235 - 2-7-2019 at 16:56

Trichoderma reesei is the typical laboratory source from which commercial cellulase is produced. There are strains that produce ridiculous quantities of enzyme.

[Edited on 3-7-2019 by UC235]