vegafelisa - 20-10-2019 at 19:33
Hi. My question is: what is the best way for extraction microrna from humans plasma? Whether trizol is suitable, or i will should use commercial kits?
Thanks
Metacelsus - 21-10-2019 at 04:02
Trizol should work, but under some conditions low GC miRNAs could be lost. Make sure to use appropriate controls. See the attached references.
Attachment: PIIS1097276512004819.pdf (350kB)
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Attachment: s12864-015-1299-5.pdf (666kB)
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Also this thread should probably be moved to the Biochemistry subforum.
[Edited on 2019-10-21 by Metacelsus]
vegafelisa - 29-10-2019 at 22:03
Thank you so much, really helpful these two attachments. I will keep your suggestion in mind next time I have any questions.