Sciencemadness Discussion Board

HPLC removal

spirocycle - 22-2-2011 at 19:30

A guy I know is willing to give me his HPLC set up, and gallons of THF for free if I can remove it safely.
I'd love to have this piece, but I have no idea how it works (specifics, I know the general theory).
Can anyone give me any advice on how to remove it safely and reinstall it?

aonomus - 22-2-2011 at 20:09

All the fittings are 10-32 threaded 1/16th inch stainless steel tubing with a fine bore. Just undo the fittings, and disconnect the modules. Software is the hardest part imo since you have to fight licensing issues and if its an old system, discontinued model support, etc.

Set the HPLC on a sturdy bench, set up a waste mobile phase container down below the instrument, set up your mobile phases, filter them. Give it a good power conditioner, etc....

Don't run your mobile phases dry, make sure you don't overtighten, just tighten until no leaks.

The hardest part will be finding replacement parts since most manufacturers only want to sell parts to companies, even if its just seals, pump pistons, new lamps (which are very, very expensive!).

To be honest if you don't *need* the HPLC, I wouldn't necessarily take it. I passed an offer for one because it was too old, non computer controlled, and no parts were likely available. You should make sure you can still service it before you take on a hard to dispose of instrument.

peach - 23-2-2011 at 01:01

If you don't want it, and are in the UK, I might be able to take it. ;)

spirocycle - 23-2-2011 at 10:21

either way, I'll still take the few gallons of THF since theyre still sealed from aldrich.

Ozone - 23-2-2011 at 13:45

Get a dionex UI-20 or ACI (use old Peaknet 5.1 that can be had for next to nothing) and convert any old HPLC, GC, etc. to accomodate computerized data acquisition.

The old spectraphysics integrators actually work quite well and the ink bladders are still sold by HP/Agilent.

Which HPLC is it? Detector type? Pump?



spirocycle - 23-2-2011 at 19:16

Im heading back on friday to get the specifics, and ill update the thread accordingly

aonomus - 25-2-2011 at 01:40

Depending on where you are, you may be able to set up an account with a 3rd party supplier of consumables. The biggest cost up front will likely be the bulb, followed by a column, followed by LC grade solvents and a filter. Let us know what you end up with, I'm curious now...

piracetam - 25-2-2011 at 20:52

I saw an LC Packings complete set (Ultimate/Switchos/Famos/Probot) sell on ebay for $207 (hardware only.) nanoflow-LC is where it's @.

just get a DAQ, find a torrent of LV, and do some low level programming to integrate the hardware.

[Edited on 26-2-2011 by piracetam]

spirocycle - 26-2-2011 at 09:39

heres the details of what they have:
7680T SFE model super critical fluid extractor
waters 150-C gel permeation chromatograph
waters 712 wisp HPLC (with autosampler)

the issue is that there is no software, and a few pumps are missing in total between the 3 machines

spirocycle - 26-2-2011 at 09:45

I cant find a manual for any of them
everytime I try to look them up, all i get is lab auction ads