Steam - 2-4-2024 at 13:05
Does anyone have any recomendations for third party labs for hire to run human cancer cell viability tests? I have contacted a couple US based
companies such as Charles river and Pacifc Biolabs, but both might be a bit outside of my budget and require huge upfront payments of roughly $20,000
or so (not counting reagent acquisition costs).
I basically want to take wild cells and cancer cells (Human LOX-IMVI melanoma cells ), treat expose to several different compounds in DMSO, and then
get a fractional viability counts (as normalized to DMSO). I think this can be done for under $10,000 (not including materials/equipment costs).
Perhaps someone here can give me a reality check. I am feeling a bit dishearted by the first two quotes.
clearly_not_atara - 3-4-2024 at 10:17
I'm pretty sure you will just kill the cells with DMSO... but anyway you should be able to run a clonogenic assay yourself, particularly if you think
you're going to cure cancer
Texium - 3-4-2024 at 10:34
It's actually quite typical to use DMSO as the vehicle in cell toxicity assays, which generally include control populations with and without exposure
to DMSO alone.
Precipitates - 3-4-2024 at 19:29
Definitely, at low concentrations you're totally fine:
My results from a cell culture course I did a few years ago (1A2 cell line).