Electra - 4-11-2015 at 13:19
I'm currently shopping for my first TLC-kit. Still hunting for some cheap plates, but in the mean time I found some reptile bulbs at a local pet store
that seek to have a peaking frequency in the 254nm range for under $10. These bulbs also output in visible regions. Would this visible light interfere
with being able to properly visualizing the TLC plate?
aga - 4-11-2015 at 14:41
Lizard Alien light is good for TLC.
Depends on what you wish to visualise, i.e. what you wish to See.
Dr.Bob - 4-11-2015 at 19:40
Using a filter to remove most of the visible light will allow you to see the fluorescence and quenching much better. You can also visualize spots on
TLCs with iodine (a little mixed with silica gel works well), ninhydrin, vanillin, permangenate and many other stains. Depends on what reagents you
can get hold of, and what you are trying to visualize.
if you are in the US, I have some various TLC plates left, most are large ones (8" x 8") which need cutting to use, but I have a few smaller ones
left. When I started, you had to either cut big ones, or make your own, but now most people buy them precut. But they can be cut and used just
fine, it does take a little practice to score and snap them, but it is doable.
AvBaeyer - 5-11-2015 at 19:48
Following up on Dr Bob's comments regarding non-uv visualization of tlc spots, the attached book is quite useful.
AvB
Attachment: Dyeing_Reagents_TLC.pdf (271kB)
This file has been downloaded 260 times
chemrox - 5-11-2015 at 22:30
Don't forget the iodine vault. Place a few crystals in a vessel with a lid appropriate for your plates.