Xrpdguy
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Hyaluronic acid
Hey guys, did anyone try ever to isolate hyaluronic acid from rooster's comb?
I have tried but something went wrong i suppose or it was a 'rooster without acid'.
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Xrpdguy
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The collected rooster combs were washed under tap
water to remove all dirt and blood stains followed by
distilled water wash. The rooster combs were weighed,
and 5 grams of rooster combs were taken in Hyaluronic acid extraction.
To start with the experiment the
Rooster combs were cut into small pieces with the help
of clean stainless steel chopper. The collected pieces of
combs were ground in an electric grinder were suspended in 100 ml acetone and were stored at 8 degrees of celsius.
After 24 hours the acetone was squeezed from the
cock's comb, and additional acetone was added. This
was repeated ten times at 24 hours interval. After the
last extraction, the remaining acetone was evaporated
in a steam of air. The dried form of
combs was then extracted ten times successively with
100 ml of 5% of Sodium acetate. Each time the viscous fluid was squeezed through several layers of
cheesecloth.
1.5 L of ethanol was added to the aqueous extract. The
precipitate was centrifuged, and dissolved in 5% sodium
acetate solution, and centrifuged again.
And now what? I just have a solution of nothing. Do you think this is the right way?
After all procedure acid should be precipitated by adding ethanol but nothing happened at all.
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TheMrbunGee
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Quote: Originally posted by Xrpdguy  | The collected rooster combs were washed under tap
water to remove all dirt and blood stains followed by
distilled water wash. The rooster combs were weighed,
and 5 grams of rooster combs were taken in Hyaluronic acid extraction.
To start with the experiment the
Rooster combs were cut into small pieces with the help
of clean stainless steel chopper. The collected pieces of
combs were ground in an electric grinder were suspended in 100 ml acetone and were stored at 8 degrees of celsius.
After 24 hours the acetone was squeezed from the
cock's comb, and additional acetone was added. This
was repeated ten times at 24 hours interval. After the
last extraction, the remaining acetone was evaporated
in a steam of air. The dried form of
combs was then extracted ten times successively with
100 ml of 5% of Sodium acetate. Each time the viscous fluid was squeezed through several layers of
cheesecloth.
1.5 L of ethanol was added to the aqueous extract. The
precipitate was centrifuged, and dissolved in 5% sodium
acetate solution, and centrifuged again.
And now what? I just have a solution of nothing. Do you think this is the right way?
After all procedure acid should be precipitated by adding ethanol but nothing happened at all. |
"Five hundred grams of frozen rooster combs yielded about 500 mg of dried HA"
You might have 5 mg of HA, If you have checked the numbers in this post of yours.. It also seems to be soluble in water, so I would not waste so much
valuable solvent.
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