reactofurnace
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Cellulase enzyme
Im trying to convert cellulose for biomass to ethanol. But first i need to convert the cellulose into a usable carbohydrate such as glucose or starch.
Does anyone know how to extract cellulase enzyme? the source I was thinking of were termites.
What y'all think?
Any other possible sources?
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BromicAcid
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Bought mine on eBay in an attempt to dissolve the leaves in my yard.
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Metacelsus
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Quote: Originally posted by reactofurnace  |
Does anyone know how to extract cellulase enzyme? the source I was thinking of were termites.
What y'all think?
Any other possible sources?
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Expressing it from a transgene in bacteria is probably the best way to go. There's quite a bit of literature on this.
Depending on the biomass, lignin may be an issue.
[Edited on 2019-7-2 by Metacelsus]
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UC235
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Trichoderma reesei is the typical laboratory source from which commercial cellulase is produced. There are strains that produce ridiculous quantities
of enzyme.
[Edited on 3-7-2019 by UC235]
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