Sciencemadness Discussion Board
Not logged in [Login ]
Go To Bottom

Printable Version  
Author: Subject: Methyl Violet questions
Sulaiman
International Hazard
*****




Posts: 3558
Registered: 8-2-2015
Location: 3rd rock from the sun
Member Is Offline


[*] posted on 31-8-2019 at 03:02
Methyl Violet questions


For no particular reason other than boredom I just ordered 25g of Methyl Violet from China via eBay
https://www.ebay.com.my/itm/25g-Methyl-Violet-Powder-Aniline...:DEcAAOSwX1JcsbRp

Methyl violet (I now discover) has different names and also has three different compounds,
the Methyl violet that I ordered has CAS# 548-62-9 which according to wikipedia
https://en.wikipedia.org/wiki/Methyl_violet
is Methyl Violet 10B aka Crystal Violet aka Gentian Violet.

The molecular formula on the container is C24H28ClN3 which would make it Methyl Violet 6B Cas# 8004-87-3 according to the wikipedia article.

I thought that I was buying crystal violet, a useful antibacterial agent,
also useful for staining bacteria etc ... but it may not be.

How can I determine which type of methyl violet I have (when it arrives)
(I heve almost no equipment or reagents at the moment) :(




CAUTION : Hobby Chemist, not Professional or even Amateur
View user's profile View All Posts By User
Pumukli
National Hazard
****




Posts: 686
Registered: 2-3-2014
Location: EU
Member Is Offline

Mood: No Mood

[*] posted on 31-8-2019 at 03:59


Afaik all triphenyl-methane dyes are cytotoxic, so it doesn't really matter which one you will receive. They will kill bacteria and probably stain cells as well.

As I see these three compounds differ only in one thing: they are either primary, secondary or tertiary amines.

Maybe with sodium nitrite you can differentiate between them.

Primary amine is diazotized and N2 can be released, phenol produced, it would switch into a chinoid form, colour change expected. Secondary makes a nitrosamine, they are usually colored (blue), the original colour of the compound may change. Tertiary amine should not make any particular reactions, I'd not expect any colour change.

At least this is my theory. :) Let's see others'!
View user's profile View All Posts By User
wg48temp9
National Hazard
****




Posts: 761
Registered: 30-12-2018
Location: not so United Kingdom
Member Is Offline


[*] posted on 31-8-2019 at 06:00


Its always a good idea to check the CAS# against the name and the formula and any other information they may have in the ad. Particularly so with dyes and pigments as they have so many names and frequently not unique ones.

The label on your violet looked identical to my violet. So I checked mine again. Its not the same label as yours and unlike your contradictory label (pic in the ad) and contradictory description in the ad, mine are consistent with the crystal violet. Of cause what it actually is apart from it being a violet dye i do not know.

I guess chromatography would distinguish them but of cause you need need known samples of both.

On the +side you can complain and perhaps get what ever it is for free.
Then order it again from a different seller without contradictory descriptions of it. Mine was from d-9845 on ebay UK.

I just checked your seller is on ebay UK too with the same incorrect ad. I am tempted to buy but i will wait to see if you get your money back and if he then does not correct the ad its fair game.






I am wg48 but not on my usual pc hence the temp handle.
Thank goodness for Fleming and the fungi.
Old codger' lives matters, wear a mask and help save them.
Be aware of demagoguery, keep your frontal lobes fully engaged.
I don't know who invented mRNA vaccines but they should get a fancy medal and I hope they made a shed load of money from it.
View user's profile View All Posts By User
Sulaiman
International Hazard
*****




Posts: 3558
Registered: 8-2-2015
Location: 3rd rock from the sun
Member Is Offline


[*] posted on 31-8-2019 at 17:11


Since posting above I've been googling a little,
and the more I read the less I know :o

It seems to me that the three forms of methyl violet (2B, 6B and 10B) are commonly confused with each other in the literature and even by suppliers, example ;
https://www.fishersci.com/shop/products/methyl-violet-2b-pur...




CAUTION : Hobby Chemist, not Professional or even Amateur
View user's profile View All Posts By User

  Go To Top