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Author: Subject: fluoresceine 113g for only £6.30
wg48temp9
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[*] posted on 28-1-2021 at 09:45
fluoresceine 113g for only £6.30


Water tracing dye available from TOOLSTATION. Probably the sodium salt.



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Housane
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[*] posted on 8-2-2021 at 04:58


Nice find, thanks



Green QD's so far

Feel free to correct grammar or incorect knknowledge. We are all learning.
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unionised
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[*] posted on 8-2-2021 at 05:12


Possibly 1% dye mixed with salt.
For £6.3 I might add some the next time I'm buying DIY stuff, and ash it to find out.

Here's the datasheet
https://cdn.aws.toolstation.com/items/coshh/19855.pdf

They also sell xylene which isn't common on the UK OTC market.


[Edited on 8-2-21 by unionised]
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[*] posted on 9-2-2021 at 03:15


I checked the safety note before I purchased it, that suggests its not cut with anything.

After purchase and before my first post I examined the powder by shaking a small quantity on a white dish to separate any different powders I could not detect any differences.

I have now attempted to recrystallize some of the powder from water, on a slow cooker hot plate (2 hours about 100C). After all the water evaporated I was left with about 1mm crystals in a brown amorphous dark brown gunge. I was not able to determine if the crystals were transparent due to the gunge.

Curiously the original powder or the gunge is not fluorescent including the acid version of fluorescein powder or any of the fluorescent dye powders I have. Apparently at high concentration and as a solid this is caused by self quenching ie resonance transfer of the excited state to nearby dye molecules.

But why don't the adjacent dye molecule produce fluorescence ?




I am wg48 but not on my usual pc hence the temp handle.
Thank goodness for Fleming and the fungi.
Old codger' lives matters, wear a mask and help save them.
Be aware of demagoguery, keep your frontal lobes fully engaged.
I don't know who invented mRNA vaccines but they should get a fancy medal and I hope they made a shed load of money from it.
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wg48temp9
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[*] posted on 10-2-2021 at 09:38


Below are some images of the self quenching effect of the fluorescence at high concentration of fluorescein.

The first image is some smears of fluorescein on white paper taken in room lighting. The brown smears are high concentrations and the yellow smears are low concentrations (small amount smeared out with a wet finger)

The second image was taken under UV light. Only the low concentration smears are fluorescing.

fluo-Capture.JPG - 44kB




I am wg48 but not on my usual pc hence the temp handle.
Thank goodness for Fleming and the fungi.
Old codger' lives matters, wear a mask and help save them.
Be aware of demagoguery, keep your frontal lobes fully engaged.
I don't know who invented mRNA vaccines but they should get a fancy medal and I hope they made a shed load of money from it.
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