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Author: Subject: Third Party Labs for Hire (Need Reality Check)
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[*] posted on 2-4-2024 at 13:05
Third Party Labs for Hire (Need Reality Check)


Does anyone have any recomendations for third party labs for hire to run human cancer cell viability tests? I have contacted a couple US based companies such as Charles river and Pacifc Biolabs, but both might be a bit outside of my budget and require huge upfront payments of roughly $20,000 or so (not counting reagent acquisition costs).

I basically want to take wild cells and cancer cells (Human LOX-IMVI melanoma cells ), treat expose to several different compounds in DMSO, and then get a fractional viability counts (as normalized to DMSO). I think this can be done for under $10,000 (not including materials/equipment costs).

Perhaps someone here can give me a reality check. I am feeling a bit dishearted by the first two quotes.




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clearly_not_atara
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[*] posted on 3-4-2024 at 10:17


I'm pretty sure you will just kill the cells with DMSO... but anyway you should be able to run a clonogenic assay yourself, particularly if you think you're going to cure cancer



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[*] posted on 3-4-2024 at 10:34


It's actually quite typical to use DMSO as the vehicle in cell toxicity assays, which generally include control populations with and without exposure to DMSO alone.



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[*] posted on 3-4-2024 at 19:29


Definitely, at low concentrations you're totally fine:

Screenshot 2024-04-04 at 10.07.06.png - 21kB

My results from a cell culture course I did a few years ago (1A2 cell line).
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[*] posted on 11-6-2024 at 14:38


Do you have to do it by a company? If not search for university labs that do this kind of research, mail the PI and offer to supply the materials and maybe a little stipend for a B. Sc. or M. Sc. thesis project.

Consumables are quite pricey, but the cost of labor and labspace is what makes contract research so expensive.

So if you can find a university that has the labspace and students that are willing to work for free that is the way to go.

Don't think of it as exploiting young enthusiasts, I am very grateful for the year of unpaid work I was allowed to do for my thesis project.

Considering DMSO, yes, it can absolutely be used in cell culture. Just make sure to include a control with DMSO and nothing else.
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[*] posted on 12-6-2024 at 07:40


Yeah, that's a typical price if you want to outsource it. My girlfriend works at a cancer lab at the Broad Institute and they make a lot of money by selling multiplexed cancer viability assays to pharma companies. (They can test several hundred cell lines at once.)



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