Empakt
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Diphenyl Carbazide Detection of unknown
Hi, I am currently using 1,5-Diphenylcarbazide to detect mercury in fish tissues. Though this reagent detects mercury, it can also detect other
metallic ions. How can I make sure it only detects the traces of mercury within the fish tissues?
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unionised
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With some difficulty. You might be able to form complexes of some of the other metals with something. eg citrate might complex copper well enough to
stop it reacting with the diphenylcarbazide.
The other way round the problem would be to reduce the mercury in the sample to the free metal, distill it, redisolve it in oxidising acid, then
measure it. Because there will only be traces of mercury the health risks from the Hg vapour shouldn't be too great but I still wouldn't want to try
it. Also, the losses would probably be considerable.
Trace metal analysis was never easy. I think you might want to look into comercial instruments for this.
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chemoleo
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I agree with unionised - quantitative chemical determination is going to be difficult, as amounts are so small and other trace ions interfere. There
are numerous analytical and qualitative ways, but they normally work with greater amounts.
I suppose it might be a good idea to first solubilise the fish tissue, and then precipiate all Hg (and other trace ions, such as PbS) with H2S. The
resultant precipitate is dissolved with HCl, and all soluble products are discarded. The remaining insoluble PbS can be redissolved in aqua regia,
and turned into i.e. Hg(Ac2)- this should not contain much insoluble ions other than Hg). Quantitative determination will have to be
spectrophotometrical (UV), also blank against a clean solution of HgAc2 to determine how the spectrum differs (as by other trace ions).
You can also use techniques such as atomic absorption spectroscopy, but I don't know if they work quantitively.
Forensic labs/ books relating to their techniques might also be a good starter.
Never Stop to Begin, and Never Begin to Stop...
Tolerance is good. But not with the intolerant! (Wilhelm Busch)
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Endo
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Mercury by AA is not usually done in an open flame or in a graphite furnace. It usually will use a heated quartz cell and metal hydride mercury vapor
generation.
In any case what I am saying is, if you want an instrumental assay for mercury you may have to find someone with an AA with the specific attachements
to assay for mercury, or a lab with a vented ICP.
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runlabrun
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http://www.cdc.gov/niosh/nmam/pdfs/6009.pdf
This is the standard NIOSH method for mercury analysis using AA. This could be adapted to fish tissues by digesting a known mass of fish tissue
followed by dilution to known volume and comparing AA results to a set of concentration standards. Your result will be mass / volume which can then be
back tracked to mass / mass and hence a % w/w mercury level can be determined (i suppose % w/w is what you would be looking for?)
-rlr
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