kevsim
Harmless
Posts: 2
Registered: 13-4-2016
Member Is Offline
Mood: No Mood
|
|
How does enzyme phenylalanine hydroxylase generate tyrosine
I have been trying to find the following information and had no luck.
Could somebody please explain -
"How does the enzyme phenylalanine hydroxylase generate
tyrosine?"
Also could somebody please explain the following -
What general type of reactions does Ligases catalyse
kevsim
|
|
|
aga
Forum Drunkard
Posts: 7030
Registered: 25-3-2014
Member Is Offline
|
|
Well, i know nothing of biochem at all, yet even I would have to ask :-
"what is phenylalanine hydroxylase generating tyrosine from ?"
and i'd probably get a clue from that.
Edit:
aren't enzyme names given by what they do ?
If so, the 'hydroxylase' part should mean something.
[Edited on 13-4-2016 by aga]
|
|
|
mnick12
Hazard to Others
Posts: 404
Registered: 30-12-2009
Location: In the lab w/ Dr. Evil
Member Is Offline
Mood: devious
|
|
Well from 5 minutes of googling it looks like hydroxylation occurs through a Fe(iv)-oxo intermediate which is formed through the cleavage of an Fe(ii)
peroxo tetrahydrobipterin intermediate.
Also Ligases, really? Just google it dude we are not doing your hw for you.
|
|
|
clearly_not_atara
International Hazard
Posts: 2694
Registered: 3-11-2013
Member Is Offline
Mood: Big
|
|
Hydroxylases in the human body mostly use the same basic idea:
* forming a complex with the target molecule such that the active site is close to an iron atom
* oxidizing said iron atom with peroxide to highly reactive/unstable FeO2+ complex
* oxygen transfer from FeO2+ to the substrate.
The FeO2+ complexes can be generated in vitro very transiently, but modern science has no molecular machinery with which to transport this
species to the desired hydroxylation site.
|
|
|
phlogiston
International Hazard
Posts: 1376
Registered: 26-4-2008
Location: Neon Thorium Erbium Lanthanum Neodymium Sulphur
Member Is Offline
Mood: pyrophoric
|
|
ligases connect two molecules together. Probably the most well known ligases are DNA ligases that connect two strands of DNA together and are commonly
used as a tool in molecular biological labs.
If we do your homework, will you please promise to just become a mailman or garbage collector, so the rest of us that is willing to make some effort
can have the nice jobs and houses with a garage large enough to put our mad scientist lab in?
-----
"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer |
|
|
kevsim
Harmless
Posts: 2
Registered: 13-4-2016
Member Is Offline
Mood: No Mood
|
|
I thank you all for the replies.
kevsim
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
