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aga
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[*] posted on 3-6-2018 at 03:35
Vicine


For over a year i've been trying to extract vicine from fava beans.

Today i picked up the latest tiny plastic pot of brown goo with the intention of throwing it out as yet another total failure.

When the pot was squeezed, it was crunchy.

Pouring off the nearly black supernatant (which had a crust on top) revealed crystals !

xtals1.jpg - 43kB xtals2.jpg - 55kB

microscope.jpg - 70kB

There isn't much, but at least there is something - finally !

Now for the scary part - recrystallising this micro-gramme amount, separating the convicine then recrystallising it again.

Happy days !




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[*] posted on 3-6-2018 at 14:12


Vicine? You got a grudge against somebody with a glucose-6-phosphatase deficiency?
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[*] posted on 3-6-2018 at 14:26


Quite the opposite.

Research is somewhat hampered by the $35,000 per-gramme cost of the stuff at analytical purity.

Marquardt et al's process is a bit beyond an amateur in the form it states.

Great googling btw.




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[*] posted on 4-6-2018 at 10:24


Do you have any Craig tubes?

They're just great for re-crystalizing small quantities with minimal loss. (with a centrifuge, but you've got one of those.)

They use them in microscale kits (that 14/10 stuff), but they must be available alone.

Did you find an MP for that stuff?
How are you planning to characterize it?




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[*] posted on 4-6-2018 at 16:37


Have you taken a look at DOI: 10.1002/jsfa.2740361210 ? The extraction looks fairly straightforward.
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[*] posted on 6-6-2018 at 11:43


Did you mean to say: "Great Googly Moogly"?

Stop being rude to people.

Folks from where I went to school, know what Favism is.

$30,000 a gram?

That might be worth the trouble.

https://www.sigmaaldrich.com/catalog/product/sial/78260?lang...

Yup!

[Edited on 6-6-2018 by zed]

[Edited on 6-6-2018 by zed]
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[*] posted on 9-6-2018 at 13:01


Quote: Originally posted by SWIM  
Do you have any Craig tubes?

No, i do not - did not even know they existed - thanks. Really good info.

I now suspect that Craig tubes are what Marquardt et al used when they said 'crystals recovered by centrifugation'.

Quote: Originally posted by UC235  
Have you taken a look at DOI: 10.1002/jsfa.2740361210 ? The extraction looks fairly straightforward.

Thanks for the reference. Yes, i've read that also.

Everything looks easy until you try and do it.

Seems to get harder at that point, especially when there is no 'No. 245 Carter Seed Cleaner' nor a 'Type 132 MP' on hand.

Quote: Originally posted by zed  
... Stop being rude to people.

I was congratulating you on your search skills, not trying to be rude.

The probability of addressing anyone with prior knowledge of vicine is vanishingly small, which i offer as an excuse for assuming it was googled.

Quote: Originally posted by zed  
... $30,000 a gram?

Closer to $40,000. Around 35,000 euros a gramme.

... not that the production of vicine could ever make anyone anyone some actual money.

It can only really be used in research, where milligrammes are more than sufficient.

It could find applications - i only found it by accident when NurdRage was making Pyrimethamine back at the NaCN stage.

Seemed to me that vicine was already mostly what he wanted to make.

If you tried to make and store a whole gramme, chances are that it would have badly degraded before a buyer was found.




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[*] posted on 10-6-2018 at 09:56


Can you state what you did? Very nice that you actually did some chemistry, but for all I know those are just funny looking NaCl crystals.

It would be preferable you explain your experiments in such detail that we could exclude other compounds forming these nice crystals.
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[*] posted on 10-6-2018 at 12:17


Perhaps it is easier to extract Vicine from Bitter Melons? If indeed, it is actually present there.

Now, I love me my Fava Beans, fer eatin', but they are just chocked full of fibrous materials and highly pigmented, seemingly oxidizable other stuff.

Never tasted them until I was 40ish, and I grew my own. Delicious! Sometimes called Windsor Beans, Broad Beans, or English Broad Beans. They were not a common food on the West Coast of the U.S.A., at least when I was a boy. In California, the Pinto, the Pinquito, and other New World Beans, reigned supreme.

Is it as it appears? That the toxic part of Vicine is not the nitrogenous ring, but rather the form of glucose it delivers as it is metabolized?

Reminds me of Solasodine (aka Curaderm), of which it is reputed, that it delivers to cancer cells, a type of sugar, that because of the cell's abnormal metabolism, kills them.

https://www.ncbi.nlm.nih.gov/pubmed/2257540

About Favas in the U.S..... Circa 1984, most people had never heard of them.

https://www.nytimes.com/1984/04/11/garden/a-bean-called-fava...





[Edited on 10-6-2018 by zed]

[Edited on 10-6-2018 by zed]

[Edited on 10-6-2018 by zed]
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[*] posted on 10-6-2018 at 13:22


The beans and the deficiency that results in favism are discussed in the popular AP Biology suggested class-read book 'Survival of the Sickest', and I suppose that many have heard of the bean and the disease from this book. This is certainly an interesting extraction, and I would love to hear how you did it Aga.




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[*] posted on 10-6-2018 at 15:44


Quote: Originally posted by Tsjerk  
Can you state what you did? Very nice that you actually did some chemistry, but for all I know those are just funny looking NaCl crystals.

Very Good point.

I was just getting used to the idea that nobody cared how anything is actually done, with just random words required instead of actual work, or details.

Over about 20 experiments, the process has been pretty much the same, using Marquardt et al's 1983 paper, but with amateur adjustments/bodges.

First the frozen beans were thawed in water, then separated from their 'testa' by hand.

The separated cotelydons were liquidised in a kitchen food blender.

(several other things were tried, the following was the last attempt)

An NaOH solution was prepared, mixed with acetone and the whizzed-up bean cotelydon mush was stirred by hand with the NaOH solution + acetone for around 15 minutes.

1 metre of 40mm diameter water pipe was used, with 4 holes drilled at one end to accept two wooden skewers to make a cross, also two holes at the opposite end to accept supporting string.

A small wad of cotton was blown from the other end to form a plug.

A small quantity (about 100g) of fine sand was then poured into the top of the pipe, with water passed through to ensure that the filtering assembly was moist.

The filter/pipe was then hung by string from a support.

The bean mush was placed in a towel and hung up so the dissolved material could drip out. This took about 2 days each time.

The pre-filtered green stuff was then put into the pipe-filter and left to filter for about 4 days.

The vast quantity of other stuff in plants makes it a slow process.

Eventually there is a lot of brown liquid in a pot.

Lacking a cyclone evaporator, it just got Boiled down, then ignored for about a month.

The Second time i did all this there were crystals - since then there were none.

That little pot with possibly-table-salt with brown goo in the photo represents a lot as far as i am concerned.

It's some crystals of something after a great many 'no-crystal' experiments.

The separation/recrystallisation isn't going well. It's a very small amount.

That part is also non-trivial it seems.




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[*] posted on 10-6-2018 at 16:15


I know a few people from SM that might offer you NMR analysis of your product, if this is indeed vicine that is quite the success. I will have to look up that paper myself. I wonder if there's info on the crystallography of vicine, it looks like you've got a pretty consistent and identifiable shape in those crystals. I'm always interested in these biological extractions.



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[*] posted on 10-6-2018 at 16:29


What are the masses of the beans and crude crystals?
What solvent u used?

[Edited on 11/06/18 by fusso]





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[*] posted on 23-6-2018 at 12:11


Quote: Originally posted by SWIM  
Do you have any Craig tubes?

They're just great for re-crystalizing small quantities with minimal loss. (with a centrifuge, but you've got one of those.)

Nope. I had nothing like a Craig tube .... until now. Thanks for mentioning them !

A bit of FreeCAD and ... Tadaaaa !

cad.jpg - 21kB

Takes about 15mins to 3D print. Took a while to scale it exactly right in the slicer (Cura) for it to 'just' fit my tubes.

It should be tight enough so that it doesn't leak until it is centrifuged, but loose enough so that liquid leaks out when you spin it up.
Simple test is to fill it with water, fit the cap, then swing it down hard in your hand.
Some drops should leak out.

There's a hole in the bottom to shove a cocktail stick in.
Turned out to be important that the bottom of the tube does not sit in liquid.

craigs.jpg - 32kB

STL file to print your own:-
Attachment: craig10.6.stl (54kB)
This file has been downloaded 49 times

[Edited on 23-6-2018 by aga]




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[*] posted on 23-6-2018 at 12:53


Awesome ! It Works !

Crystals in brown goo

inpot.jpg - 44kB

Transfer to plastic test tube (my glass ones do not have very straight sides - there's a lip)

transfer.jpg - 39kB

3D printed in PLA 'Craig' adapter fitted plus cocktail stick

lollipop.jpg - 43kB

Assembly after about 3 minutes of cranking the handle on my Indian centrifuge

after.jpg - 34kB

Remarkably the crystals were free in the tube, as if dried overnight.
I just flicked the tube once and they all fell out onto a watch glass.

dish.jpg - 43kB

They still look like brown sugar, and are likely highly contaminated with NaCl.
Maybe that's what they actually are, seeing as the pH was adjusted with NaOH + HCl.

Either way, i'm really impressed with this Craig Tube thing for separating crystals from the mother liquor.

It's just so fast !




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[*] posted on 23-6-2018 at 13:48


Forgot to mention that it was the Small pot that got whizzed.

Big pot tomorrow, probably 5 batches in all.




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[*] posted on 24-6-2018 at 06:45


After a lot of cranking the handle on the centrifuge today, all the crystals were separated from the thick brown mother liquor.

Vicine is highly soluble at a pH of 1 wheras convicine is not, so i decided to add 20% HCl to the crystals to separate the two substances at this point

A tiny portion refused to dissolve, so they should be mostly convicine, certainly not NaCl.
These were separated by decantation and placed in an eppendorf tube, a craig adapter fitted, then centrifuged.

eppy.jpg - 34kB

These crystals were washed twice with HCl and recentrifuged, yielding ~100mg of brown crystals.

yield.jpg - 52kB

To the naked eye they appear square-ish.
Under a microscope they have an odd rounded, almost 'organic' look

2018-06-24-163059.jpg - 45kB

The HCl portion separated earlier should contain the vicine, so it was filtered, pH adjusted to ~7 with NaOH, then split between 9 plastic test tubes with caps, which were all placed in a cool box with plenty of ice.

Hopefully crystals will appear, which can then be directly centrifuged out with no need for another transfer.

[Edited on 24-6-2018 by aga]




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[*] posted on 24-6-2018 at 09:00


Quote: Originally posted by aga  
... probably 5 batches in all.


How many beans make five?
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[*] posted on 24-6-2018 at 09:05


About 3kg of frozen beans in this latest batch.

TBH i can't exactly remember.





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[*] posted on 11-7-2018 at 17:45


Congratulations Aga, that's some great work! Do you have anything you plan on doing with them? Perhaps a metal-complex?



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