polymer
Harmless
Posts: 36
Registered: 9-12-2006
Location: in deep space
Member Is Offline
Mood: unsteady
|
|
Sequencing DNA
Can someone tell me how to sequence DNA?
E/m*c^2
|
|
|
XxDaTxX
Hazard to Self
Posts: 66
Registered: 12-3-2006
Member Is Offline
Mood: No Mood
|
|
Nowadays you send it out, or have your undergrads run those hideous gels for you. =]
|
|
|
Maya
Hazard to Others
Posts: 263
Registered: 3-10-2006
Location: Mercury
Member Is Offline
Mood: molten
|
|
your target sequence should be no more than 1000 base pairs long. First you aneal your oligonucleotide primers to each end of your single stranded
DNA. Then using taq, radioactive phosporus and dT dC dA dG you fill in varying lengths of your sequence and finally run that on an acrylamide gel and
read the autoradiograph sequence you get after putting some film on the gel for 12 hours in the -80 freezer
piece of cake!
Or you can just send your sample off to the core and let them do it! 
[Edited on 14-9-2007 by Maya]
\"Prefiero ser yo extranjero en otras patrias, a serlo en la mia\"
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
