Difference between revisions of "Rhodamine B"

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[[Category:Triarylmethane dyes]]

Latest revision as of 19:26, 23 November 2023

Rhodamine B
Names
IUPAC name
9-(2-Carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride
Other names
Basic Violet 10
Brilliant Pink B
C.I. 45170
C.I. Pigment Violet 1
Rhodamine 610
Properties
C28H31ClN2O3
Molar mass 479.02 g/mol
Appearance Red to violet powder
Odor Odorless
Melting point 210–211 °C (410–412 °F; 483–484 K) (decomposition)
Boiling point Decomposes
8 to 15 g/L (20 °C)
Solubility Soluble in methanol, ethanol
Vapor pressure ~0 mmHg
Hazards
Safety data sheet Sigma-Aldrich
Related compounds
Related compounds
Fluorescein
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport, or more commonly as staining fluorescent dye in biology.

Properties

Chemical

Rhodamine B can exist in equilibrium between two forms: an "open"/fluorescent form and a "closed"/nonfluorescent spirolactone form. The "open" form dominates in acidic condition while the "closed" form is colorless in basic condition.

Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium

Physical

Rhodamine B is a reddish solid, soluble in water and alcohol.

Availability

Rhodamine B is sold by chemical suppliers.

Preparation

Rhodamine B can be prepared by reacting phthalic anhydride with m-diethylaminophenol.

Projects

  • Stain dye
  • Fluorescent dye
  • Make Opera Rose

Handling

Safety

Rhodamine B is harmful if ingested in large amounts. In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label. Rhodamine B is notorious for its staining properties, even minute traces will stain.

Storage

In closed airtight bottles.

Disposal

Should be strongly diluted with water and poured down the drain.

References

Relevant Sciencemadness threads