rannyfash - 12-8-2014 at 17:02
I apologise if the answer is available online, i have been unable to find a definite answer, Do amino acids precipitate out of aqueous solution at
their respective isoelectric points? if not is there a method other than chromatography for the separation of large quantities of amino acids?
JAVA - 20-8-2014 at 12:26
If their pI value is reached they might form a precipitate with a saturated ammonium sulphate solution and diluted HCl.
If not there are options to get them out of the solution, amino acids are very soluble in water but maybe less in water/acetone or another solvent
that is miscible in water, maybe.
Ninhydride is used to find out if the precipitate are really amino acids. The pI of every amino acid is different.
Flash chromatography looks the easiest way to separate them, but if this isn't allowed then gel electrophoresis might be a method to separate them.
btw: I think that it's tuff to get cysteine in the pure form because of oxidation.
rannyfash - 30-8-2014 at 13:59
thanks, those methods are used to precipitate proteins and i think they might work but i cant find anything on the internet (there must be something
but i cant find it), my aim is for the end product to be edible, ill give it a try when im not so busy