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Author: Subject: Inexpensive DIY capillary electrophoresis or other separation techniques?
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shocked.gif posted on 28-11-2025 at 07:37
Inexpensive DIY capillary electrophoresis or other separation techniques?


Hi,

I'd like to know what is the simplest and cheapest approach to do capillary electrophoresis (or any other separation technique).
Specifically thinking about separation of phytochemicals.

I noticed this paper was quoted here in the forums a while ago:
An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection

However this one was published more recently:
Low-cost automated capillary electrophoresis instrument assembled from commercially available parts

This one instead is just a broader overview which however I'm getting lost in: Low-cost and open-source strategies for chemical separations

To perform CE, could I simply use:
1 glass capillary
2 beakers
2 graphite electrodes
a power source

until the desired phytochemical has migrated from one beaker to the other, and stop just before other phytochemicals start migrating?

Thank you
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Metacelsus
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[*] posted on 29-11-2025 at 04:38


Hmm, it sounds like you're trying to use capillary electrophoresis as a preparative (rather than analytical) separation technique? Typically only tiny amounts are loaded onto the capillary and I don't think it will scale up very well.



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[*] posted on 29-11-2025 at 06:05


Quote: Originally posted by Metacelsus  
Hmm, it sounds like you're trying to use capillary electrophoresis as a preparative (rather than analytical) separation technique? Typically only tiny amounts are loaded onto the capillary and I don't think it will scale up very well.


Yes I'd use it to separate rather than just analyze. Which other techniques would you suggest?

Thank you
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