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Author: Subject: requesting a calculation issue
calamus
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[*] posted on 6-7-2010 at 13:18
requesting a calculation issue


i hope someone could explain me when I'm analyzing a plant material for carbohydrates , lipids , phenolic compounds , flavonoids or protien "total"
how i express these data as total carbohydrates was 20 mg/1g DW (dry weight) and some times FW (fresh weight)
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psychokinetic
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[*] posted on 6-7-2010 at 13:34


You just did.

20mg/g DW or
20mg/g FW or
20mg/g average concentration over FW+DW etc.

As long as you state what FW and DW mean, and how they are decided upon.




“If Edison had a needle to find in a haystack, he would proceed at once with the diligence of the bee to examine straw after straw until he found the object of his search.
I was a sorry witness of such doings, knowing that a little theory and calculation would have saved him ninety per cent of his labor.”
-Tesla
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calamus
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[*] posted on 7-7-2010 at 00:45


hi psychokinetic ,
thanks for replying yes i said it is represented as FW or DW
but i wanna understand how we reach these values from say 1 g of plant material fresh or dry and extracting it by a solvent say 20 ml and then measuring it on spectrophotometer and obtaining concentration from standard curve we prepared
then expressing the data as values mentioned 20 mg/g
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psychokinetic
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[*] posted on 7-7-2010 at 13:28


Oh, I think I see what you mean. Sorry!

You want to show how 1g/20ml of test solution comes up with the end result of 20mg/g x?

Hmm. Unfortunately I'm not sure on that one, though it may be easier to figure out if I was actually doing it :P




“If Edison had a needle to find in a haystack, he would proceed at once with the diligence of the bee to examine straw after straw until he found the object of his search.
I was a sorry witness of such doings, knowing that a little theory and calculation would have saved him ninety per cent of his labor.”
-Tesla
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calamus
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[*] posted on 14-7-2010 at 10:14


thanks psychokinetic
i'll will try to resolve that
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