user1007
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Registered: 20-5-2015
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extracting phytosterols from vegetable oils?
Saw an interesting post about chemical oxidation of cholesterol.
Wondered if the same would apply to phyosterol, curious about trying it.
Then the questions remained of an easy source of phyosterol, nutritiondata.self.com quites rice bran oil as have 1190mg of phytosterols in a 100g
serving, which is about the highest I could find, and a reasonable amount.
Is extraction as simple continuously agitating ethanol in cold oil, separating and drying out the ethanol? Looking for a shove in the right direction
here.
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Endo
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Registered: 5-1-2006
Location: USA
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User 1007,
I have some experience with phytosterols their separation and analysis. Typically in an oil you will find the various phytosterols as fatty acid
esters, this makes the very hard to extract from the vegetable oil matrix in their typical state. I suggest you search some of the literature and see
how they did their separations. When asked to analyze the phytosterols (GC-FID) I would saponify the mixture with alcoholic KOH or NaOH, then form a
more volatile trimethylsilyl ester using MSTFA. This allowed analysis of the specific phytosterols present without having 'families' of the same
phytosterol but with differing length fatty acid chains for the ester group.
In your case I suggest you read this paper: http://link.springer.com/article/10.1007%2FBF02856537 The section headed saponification copied below outlines what should be a workable process.
You will still have a mixture of different phytosterols once you get to the end product.
*Snip*
Saponification
30g of corn oil foots and 150 ml of ethanolic KOH
(1 N) were placed to the 1 L round bottomed flask
equipped with reflex condenser. After the reaction
mixture was stirred for 2 hours at 70~ it was cooled
to room temperature and diluted with 100 ml of water
and extracted with 100 ml of dichloromethane, 4
times. The combined organic layer was washed with
100 ml of 0.1 N NaOH aq. solution and 100 ml of
water, dried over anhydrous magnesium sulfate, and
evaporated under reduced pressure to give dark brown
residue. This insaponifiable was placed to the 50 ml
of round bottomed flask with condenser containing
20 ml of ethanol, and heated to 80~ with good stirring
until it was dissolved completely. After cooling to
room temperature, the crystalline precipitate was
filtered and dried under vacuum.
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