dennisfrancisblewettiii
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Location: Madison, Wisconsin
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Lab work (orgo lab): Oxidizing leucomethylene blue?
I have a part of the lab that asks how I might about about analyzing a colorless fraction of leucomethylene blue after I elute methylene blue with
solvents (i'm eluting both methyl orange and methylene blue). The solvents I am choosing are hexane and toulene. I expect that it will elute, but I'm
not sure what to oxidize it with. I suspect a lot of the solution will look colorless, so I won't be able to know when it has eluted.
In the lab, I believe there will be these solutions and compounds, water, methanol, ethanol, 1-propanol, acetone, toulene, hexane, and Na2SO4.
Can I use any of these as oxidizing agents?
How should I go about oxidizing the leucomethylene blue?
Thanks.
- Genecks
[Edited on 14-9-2011 by Genecks]
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Ozone
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We use it (reduced with glucose) as an oxygen indicator in anaerobic fermentations. In protic media, oxygen (from the air) readily oxidizes it to give
the blue species. See:
http://www.udel.edu/pchem/C446/Experiments/exp7.pdf
-Anyone who never made a mistake never tried anything new.
--Albert Einstein
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dennisfrancisblewettiii
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Registered: 16-12-2005
Location: Madison, Wisconsin
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Are you saying that being in air long enough should give it its color back?
I'm thinking maybe air is the oxidizing agent and all I have to do is shake the test tube or stir the elute in a beaker, but I'm not sure that is the
case, because it seems that leucomethylene blue needs some kind of chemical to change the bonds. I could be wrong, though.
I read about the blue bottle experiment, and it seems to have other chemicals inside of it other than the methylene blue; thus, I beleive that for my
experiment, I'll need to add some other chemical and shake it in an environment with air.
[Edited on 14-9-2011 by Genecks]
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