andre178
Hazard to Self
Posts: 61
Registered: 11-12-2009
Location: San Diego, CA
Member Is Offline
Mood: pacified
|
|
equivalents of salt
Hello, beginner question, a patent synthesis calls for 4.5 equivalents of Fremy's salt to add to dihydroethidium (60 uM) in 0.1 M phosphate buffer
under stirring.
Can someone ellucidate what the 4.5 equivalents refers to, I'm not sure, is it potassium to the rest of the salt or is this something else?
|
|
|
Ozone
International Hazard
Posts: 1269
Registered: 28-7-2005
Location: Good Olde USA
Member Is Offline
Mood: Integrated
|
|
I assume equivalent to nitrosyl radical? It's use can be seen here:
http://www.orgsyn.org/orgsyn/orgsyn/prepContent.asp?prep=cv6...
The mechanistic similarity with superoxide is given in the attached paper.
An interesting comparison with TEMPO (et al.) is given in the papers referring to DPPH and roasted coffee, which are also attached.
Cheers,
O3
Attachment: Zielonka et al 2005_Fremys salt Mechanism.pdf (378kB)
This file has been downloaded 1008 times
Attachment: Ionita 2003 DPPH fremys tempo.pdf (156kB)
This file has been downloaded 691 times
Attachment: Cammerer and Kroh 2006 antioxidants in coffee TEMPO etc.pdf (349kB)
This file has been downloaded 518 times
-Anyone who never made a mistake never tried anything new.
--Albert Einstein
|
|
|
DJF90
International Hazard
Posts: 2266
Registered: 15-12-2007
Location: At the bench
Member Is Offline
Mood: No Mood
|
|
4.5 eq. of Fremy's salt means use 4.5 moles of it per mole of substrate.
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
