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Author: Subject: Cellulase enzyme
reactofurnace
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[*] posted on 1-7-2019 at 19:29
Cellulase enzyme


Im trying to convert cellulose for biomass to ethanol. But first i need to convert the cellulose into a usable carbohydrate such as glucose or starch.

Does anyone know how to extract cellulase enzyme? the source I was thinking of were termites.

What y'all think?

Any other possible sources?

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BromicAcid
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[*] posted on 1-7-2019 at 19:48


Bought mine on eBay in an attempt to dissolve the leaves in my yard.




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Metacelsus
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[*] posted on 1-7-2019 at 23:05


Quote: Originally posted by reactofurnace  

Does anyone know how to extract cellulase enzyme? the source I was thinking of were termites.

What y'all think?

Any other possible sources?



Expressing it from a transgene in bacteria is probably the best way to go. There's quite a bit of literature on this.

Depending on the biomass, lignin may be an issue.

[Edited on 2019-7-2 by Metacelsus]




As below, so above.
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UC235
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[*] posted on 2-7-2019 at 16:56


Trichoderma reesei is the typical laboratory source from which commercial cellulase is produced. There are strains that produce ridiculous quantities of enzyme.

[Edited on 3-7-2019 by UC235]
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