Steam
Hazard to Others
Posts: 238
Registered: 25-3-2014
Location: Minnesota
Member Is Offline
Mood: Triple Point
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Third Party Labs for Hire (Need Reality Check)
Does anyone have any recomendations for third party labs for hire to run human cancer cell viability tests? I have contacted a couple US based
companies such as Charles river and Pacifc Biolabs, but both might be a bit outside of my budget and require huge upfront payments of roughly $20,000
or so (not counting reagent acquisition costs).
I basically want to take wild cells and cancer cells (Human LOX-IMVI melanoma cells ), treat expose to several different compounds in DMSO, and then
get a fractional viability counts (as normalized to DMSO). I think this can be done for under $10,000 (not including materials/equipment costs).
Perhaps someone here can give me a reality check. I am feeling a bit dishearted by the first two quotes.
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clearly_not_atara
International Hazard
Posts: 2701
Registered: 3-11-2013
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Mood: Big
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I'm pretty sure you will just kill the cells with DMSO... but anyway you should be able to run a clonogenic assay yourself, particularly if you think
you're going to cure cancer
[Edited on 04-20-1969 by clearly_not_atara]
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Texium
Administrator
Posts: 4522
Registered: 11-1-2014
Location: Salt Lake City
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Mood: PhD candidate!
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It's actually quite typical to use DMSO as the vehicle in cell toxicity assays, which generally include control populations with and without exposure
to DMSO alone.
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Precipitates
Harmless
Posts: 42
Registered: 4-12-2023
Location: SE Asia
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Definitely, at low concentrations you're totally fine:
My results from a cell culture course I did a few years ago (1A2 cell line).
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