Rhodamine B
| Names | |
|---|---|
| IUPAC name
9-(2-Carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride
| |
| Other names
Basic Violet 10
Brilliant Pink B C.I. 45170 C.I. Pigment Violet 1 Rhodamine 610 | |
| Properties | |
| C28H31ClN2O3 | |
| Molar mass | 479.02 g/mol |
| Appearance | Red to violet powder |
| Odor | Odorless |
| Melting point | 210–211 °C (410–412 °F; 483–484 K) (decomposition) |
| Boiling point | Decomposes |
| 8 to 15 g/L (20 °C) | |
| Solubility | Soluble in methanol, ethanol |
| Vapor pressure | ~0 mmHg |
| Hazards | |
| Safety data sheet | Sigma-Aldrich |
| Related compounds | |
| Related compounds
|
Fluorescein |
| Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport, or more commonly as staining fluorescent dye in biology.
Contents
Properties
Chemical
Rhodamine B can exist in equilibrium between two forms: an "open"/fluorescent form and a "closed"/nonfluorescent spirolactone form. The "open" form dominates in acidic condition while the "closed" form is colorless in basic condition.
Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium
Physical
Rhodamine B is a reddish solid, soluble in water and alcohol.
Availability
Rhodamine B is sold by chemical suppliers.
Preparation
Rhodamine B can be prepared by reacting phthalic anhydride with m-diethylaminophenol.
Projects
- Stain dye
- Fluorescent dye
- Make Opera Rose
Handling
Safety
Rhodamine B is harmful if ingested in large amounts. In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label. Rhodamine B is notorious for its staining properties, even minute traces will stain.
Storage
In closed airtight bottles.
Disposal
Should be strongly diluted with water and poured down the drain.
